Overview

  • Product name

  • Description

    Goat polyclonal to Parvalbumin
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Macaque monkey
  • Immunogen

    Synthetic peptide:

    GVDEFSTLVAES

    , corresponding to C terminal amino acids 98-109 of Human Parvalbumin

  • Positive control

    • Human Brain lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).

Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. 

ELISA 1/32000.
IHC-Fr Use at an assay dependent concentration.

Target

Images

  • Anti-Parvalbumin antibody (ab32895) at 0.3 µg/ml + Human Brain lysate at 35 µg

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3µg/ml. 

References

This product has been referenced in:

  • Eshima H  et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep 5:N/A (2017). Read more (PubMed: 28408640) »
  • Iaccarino HF  et al. Gamma frequency entrainment attenuates amyloid load and modifies microglia. Nature 540:230-235 (2016). Read more (PubMed: 27929004) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10mM Tween 20 0.5%
Permeabilization
Yes - Triton 0.75%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Mar 11 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - TRITON 1%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Mar 11 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - triton 0.75%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted Jul 19 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (frozen brain section)
Permeabilization
No
Specification
frozen brain section
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 07 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (hippocampus))
Permeabilization
No
Specification
Brain (hippocampus)
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 19 2012

Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 15 2011

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