Overview

  • Product name

    Anti-Parvalbumin antibody [EPR13091]
    See all Parvalbumin primary antibodies
  • Description

    Rabbit monoclonal [EPR13091] to Parvalbumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Parvalbumin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P20472

  • Positive control

    • Recombinant Human Parvalbumin protein (ab101107) can be used as a positive control in WB. Human cerebellum tissue lysate; Human cerebellum tissue; Human chromophobe carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13091
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P 1/250 - 1/500.
IP 1/40.
IHC-Fr 1/100.

Target

Images

  • ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

    Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).

  • ab181086 staining Parvalbumin in rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/2000). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

    Positive staining on Purkinje cells and neurons of the molecular layer in rat cerebellum (PMID: 22561329).

  • ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/2000). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

    Positive staining on mouse skeletal muscle (PMID: 1837548).

  • ab181086 staining Parvalbumin in mouse cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/2000). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

    Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).

    The section was incubated with ab181086 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

    Positive staining on skeletal muscles (PMID: 7604022).

  • ab181086 (purified) at 1:30 dilution (1µg) immunoprecipitating Parvalbumin in Human cerebellum lysate.

    Lane 1: Human cerebellum lysate 10μg
    Lane 2 (+): ab181086 & Human cerebellum lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181086 in Human cerebellum lysate
    For western blotting, VeriBlot for IP Detection reagent (HRP) (ab131366) was used at 1:5000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate prepared using RIPA lysis method
    Lane 2 : Rat skeletal muscle lysate prepared using RIPA lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa


    Exposure time: 75 seconds
  • All lanes : Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution

    Lane 1 : Mouse cerebellum lysate prepared using RIPA lysis method
    Lane 2 : Mouse cerebellum lysate prepared using 1% SDS hot lysis method
    Lane 3 : Rat cerebellum lysate prepared using 1% SDS hot lysis method
    Lane 4 : Human brain lysate prepared using RIPA lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 12 kDa
    Observed band size: 12 kDa


    Exposure time: 3 minutes


    The rat cerebellum lysate in lane 3 is prepared by 1%SDS hot lysis method. This antibody can work in RIPA and 1% SDS hot lysates in WB.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section.

  • Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Parvalbumin with ab181086 at a 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human chromophobe carcinoma tissue labeling Parvalbumin with ab181086 at a 1/500 dilution.

  • Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/10000 dilution + Human cerebellum tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa

  • Western blot analysis on immunoprecipitation pellet from Human cerebellum tissue lysate labeling Parvalbumin with ab181086 at 1/40 dilution.

References

ab181086 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Coronal brain sections)
Specification
Coronal brain sections

Abcam user community

Verified customer

Submitted Mar 21 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up