Recombinant Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2174] to PARVA (phospho S8)
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PARVA (phospho S8) antibody [EPR2174]
See all PARVA primary antibodies -
Description
Rabbit monoclonal [EPR2174] to PARVA (phospho S8) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 cell lysate. HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2174 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/250.
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WB |
1/2500 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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Notes |
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ICC/IF
1/100 - 1/250. |
WB
1/2500 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
Target
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Function
Probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Plays a role in ciliogenesis. -
Tissue specificity
Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. -
Sequence similarities
Belongs to the parvin family.
Contains 2 CH (calponin-homology) domains. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Cytoplasm > cytoskeleton. Constituent of focal adhesions. - Information by UniProt
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Database links
- Entrez Gene: 55742 Human
- Entrez Gene: 57342 Mouse
- Entrez Gene: 57341 Rat
- Omim: 608120 Human
- SwissProt: Q9NVD7 Human
- SwissProt: Q9EPC1 Mouse
- SwissProt: Q9HB97 Rat
- Unigene: 432914 Human
see all -
Alternative names
- Actopaxin antibody
- Alpha parvin antibody
- Alpha-parvin antibody
see all
Images
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All lanes : Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409) at 1/5000 dilution
Lane 1 : A549 cell lysate
Lane 2 : A549 cell lysate treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunofluorescent staining of HeLa cells using ab79409 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab79409 has not yet been referenced specifically in any publications.