Anti-PASD1 antibody (ab224363)

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Overview

  • Product name

    Anti-PASD1 antibody
    See all PASD1 primary antibodies

  • Description

    Rabbit polyclonal to PASD1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PASD1 aa 84-231.
    Sequence:

    EEKDEVYQKIILKFPLLNSETHIEFCCHLKRGNVEHGDSSAYENVKFIVN VRDICNEFPVVFSGLFSSHLCADFAACVPQEDRLYLVGNVCILRTQLLQQ LYTSKAVSDEAVLTQDSDEEPFVGELSSSQGQRGHTSMKAVYVEPAAA


    Database link: Q8IV76

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC: Human testis tissue. ICC/IF: PC-3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May function as a transcription factor.

  • Tissue specificity

    Testis-specific. Found in histologically normal tissues from patients with uterus, lung and small intestine cancers. Widespread expression seen in solid tumors and diffuse large B-cell lymphoma (DLBCL)-derived cell lines. Isoform 2 is expressed in all DLBCL-derived cell lines, while isoform 1 is preferentially expressed in cell lines derived from non-germinal center DLBCL.

  • Sequence similarities

    Contains 1 PAS (PER-ARNT-SIM) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cancer/testis antigen 63 antibody
      • CT63 antibody
      • OX-TES-1 antibody
      • PAS domain containing 1 antibody
      • PAS domain-containing protein 1 antibody
      • PASD1 antibody
      • PASD1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PASD1 antibody (ab224363)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PASD1 antibody (ab224363)

      Paraffin-embedded human testis tissue stained for PASD1 with ab224363 (1/200) in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-PASD1 antibody (ab224363)
      Immunocytochemistry/ Immunofluorescence - Anti-PASD1 antibody (ab224363)

      PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for PASD1 (green) using ab224363 (4 µg/ml) in ICC/IF.

    References

    ab224363 has not yet been referenced specifically in any publications.

    Publishing research using ab224363? Please let us know so that we can cite the reference in this datasheet.

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