Anti-NPAS1 antibody (ab183655)
Key features and details
- Rabbit polyclonal to NPAS1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NPAS1 antibody -
Description
Rabbit polyclonal to NPAS1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human NPAS1 aa 116-398. The exact sequence is proprietary.
Database link: Q99742 -
Positive control
- U87-MG cells and cell lysates; IMR32 and SK-N-AS cell lysates; mouse liver tissue; mouse middle brain tissue; rat fore brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 63 kDa.
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ICC/IF | (1) |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 63 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
PASD5 is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of PASD5 is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 4861 Human
- Entrez Gene: 18142 Mouse
- Entrez Gene: 308387 Rat
- Omim: 603346 Human
- SwissProt: Q99742 Human
- SwissProt: P97459 Mouse
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Alternative names
- Basic-helix-loop-helix-PAS protein MOP5 antibody
- bHLHe11 antibody
- Class E basic helix loop helix protein 11 antibody
see all
Images
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All lanes : Anti-NPAS1 antibody (ab183655) at 1/1000 dilution
Lane 1 : U87-MG whole cell lysate/extract
Lane 2 : IMR32 whole cell lysate/extract
Lane 3 : SK-N-AS whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 63 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling NPAS1 with ab183655 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse middle brain tissue labeling NPAS1 with ab183655 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded rat fore brain tissue labeling NPAS1 with ab183655 at 1/500 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed U87-MG cells labeling NPAS1 with ab183655 at 1/500 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183655 has been referenced in 1 publication.
- Michaelson JJ et al. Neuronal PAS Domain Proteins 1 and 3 Are Master Regulators of Neuropsychiatric Risk Genes. Biol Psychiatry 82:213-223 (2017). PubMed: 28499489