Anti-PASK antibody (ab114988)
Key features and details
- Rabbit polyclonal to PASK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PASK antibody
See all PASK primary antibodies -
Description
Rabbit polyclonal to PASK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
-
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 143 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Tissue specificity
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on Thr-1161 and Thr-1165. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 23178 Human
- Omim: 607505 Human
- SwissProt: Q96RG2 Human
- Unigene: 397891 Human
-
Alternative names
- DKFZP434O051 antibody
- DKFZp686P2031 antibody
- hPASK antibody
see all
Images
-
All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 3 minutes -
Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 mminutes.
All lanes : Anti-PASK antibody (ab114988) at 0.4 µg/ml
Lane 1 : HeLa cell lysate, IP with ab114988 at 6 µg per mg lysate
Lane 2 : HeLa cell lysate, IP with alternative PASK antibody
Lane 3 : HeLa cell lysate, IP with control IgG
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab114988 has not yet been referenced specifically in any publications.