Key features and details
- Rabbit polyclonal to PASK - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PASK antibody - C-terminal
See all PASK primary antibodies
DescriptionRabbit polyclonal to PASK - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to a region within C terminal amino acids 982-1227 of Human PASK (Uniprot ID: Q96RG2).
- Mouse brain whole cell lysate; HeLa cells; Human NCIN87 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 143 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityUbiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on Thr-1161 and Thr-1165.
- Information by UniProt
- DKFZP434O051 antibody
- DKFZp686P2031 antibody
- hPASK antibody
Anti-PASK antibody - C-terminal (ab154053) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 143 kDa
5% SDS PAGE
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling PASK with ab154053 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue, labeling PASK with ab154053 at 1/500 dilution.
ab154053 has not yet been referenced specifically in any publications.