Key features and details
- Rabbit polyclonal to Pat1b
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pat1b antibody
See all Pat1b primary antibodies
DescriptionRabbit polyclonal to Pat1b
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human Pat1b aa 425-475.
Database link: NP_689929.2
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
Previously labelled as PATL1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 1 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
Sequence similaritiesBelongs to the PAT1 family.
DomainThe region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
Cellular localizationCytoplasm > P-body.
- Information by UniProt
- hPat1b antibody
- OK/KNS-cl.5 antibody
- PAT1-like protein 1 antibody
All lanes : Anti-Pat1b antibody (ab124257) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 1 kDa
Exposure time: 3 minutes
ab124257 at 1 µg/ml staining Pat1b in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
ab124257 has been referenced in 2 publications.