Anti-Pat1b antibody (ab124257)

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Overview

  • Product name
    Anti-Pat1b antibody
    See all Pat1b primary antibodies
  • Description
    Rabbit polyclonal to Pat1b
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Pat1b aa 425-475.
    Database link: NP_689929.2

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysate (ab7899)
  • General notes

    Previously labelled as PATL1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab124257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the PAT1 family.
  • Domain
    The region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
    The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • hPat1b antibody
      • OK/KNS-cl.5 antibody
      • PAT1-like protein 1 antibody
      • Pat1b antibody
      • PATL1 antibody
      • PATL1_HUMAN antibody
      • protein associated with topoisomerase II homolog 1 antibody
      • Protein PAT1 homolog 1 antibody
      • Protein PAT1 homolog b antibody
      see all

    Images

    • Western blot - Anti-Pat1b antibody (ab124257)
      Western blot - Anti-Pat1b antibody (ab124257)
      All lanes : Anti-Pat1b antibody (ab124257) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 1 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-Pat1b antibody (ab124257)
      Immunoprecipitation - Anti-Pat1b antibody (ab124257)

      ab124257 at 1 µg/ml staining Pat1b in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

    References

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