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  1. Link

    pat1b-antibody-ab124257.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Scaffold Proteins
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Anti-Pat1b antibody (ab124257)

  • Datasheet
Submit a review Submit a question References (2)

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Western blot - Anti-Pat1b antibody (ab124257)
  • Immunoprecipitation - Anti-Pat1b antibody (ab124257)

Key features and details

  • Rabbit polyclonal to Pat1b
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Pat1b antibody
  • Description

    Rabbit polyclonal to Pat1b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Pat1b aa 425-475.
    Database link: NP_689929.2

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899)
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HCV
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV).
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the PAT1 family.
  • Domain

    The region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
    The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain.
  • Cellular localization

    Cytoplasm > P-body.
  • Target information above from: UniProt accession Q86TB9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 219988 Human
    • Entrez Gene: 225929 Mouse
    • SwissProt: Q86TB9 Human
    • SwissProt: Q3TC46 Mouse
    • Unigene: 591960 Human
    • Unigene: 42711 Mouse
    • Alternative names

      • hPat1b antibody
      • OK/KNS-cl.5 antibody
      • PAT1-like protein 1 antibody
      • Pat1b antibody
      • PATL1 antibody
      • PATL1_HUMAN antibody
      • protein associated with topoisomerase II homolog 1 antibody
      • Protein PAT1 homolog 1 antibody
      • Protein PAT1 homolog b antibody
      see all

    Images

    • Western blot - Anti-Pat1b antibody (ab124257)
      Western blot - Anti-Pat1b antibody (ab124257)
      All lanes : Anti-Pat1b antibody (ab124257) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : 293T whole cell lysate at 50 µg
      Lane 4 : Jurkat whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 1 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-Pat1b antibody (ab124257)
      Immunoprecipitation - Anti-Pat1b antibody (ab124257)

      ab124257 at 1 µg/ml staining Pat1b in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab124257? Please let us know so that we can cite the reference in this datasheet.

    ab124257 has been referenced in 2 publications.

    • Gatica D  et al. The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy. Mol Cell 73:314-324.e4 (2019). PubMed: 30527663
    • D'Lima NG  et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). PubMed: 27918561

    Customer reviews and Q&As

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