Anti-Pat1b antibody (ab124257)
Key features and details
- Rabbit polyclonal to Pat1b
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pat1b antibody -
Description
Rabbit polyclonal to Pat1b -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Pat1b aa 425-475.
Database link: NP_689929.2 -
Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| Notes |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 1 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
RNA-binding protein involved in deadenylation-dependent decapping of mRNAs, leading to the degradation of mRNAs. Acts as a scaffold protein that connects deadenylation and decapping machinery. Required for cytoplasmic mRNA processing body (P-body) assembly. In case of infection, required for translation and replication of hepatitis C virus (HCV). -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the PAT1 family. -
Domain
The region A, also named N-term, mediates the interaction with DDX6/RCK and is required for cytoplasmic mRNA processing body assembly.
The region C, also named Pat-C, is required for RNA-binding and mediates the binding with the Lsm-containing SMN-Sm protein complex and the decapping machinery. It folds into an alpha-alpha superhelix, exposing conserved and basic residues on one side of the domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 219988 Human
- Entrez Gene: 225929 Mouse
- SwissProt: Q86TB9 Human
- SwissProt: Q3TC46 Mouse
- Unigene: 591960 Human
- Unigene: 42711 Mouse
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Alternative names
- hPat1b antibody
- OK/KNS-cl.5 antibody
- PAT1-like protein 1 antibody
see all
Images
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Western blot - Anti-Pat1b antibody (ab124257)All lanes : Anti-Pat1b antibody (ab124257) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 1 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Pat1b antibody (ab124257)ab124257 at 1 µg/ml staining Pat1b in HeLa whole cell lysate immunoprecipitated using ab124257 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab124257 has been referenced in 2 publications.
- Gatica D et al. The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy. Mol Cell 73:314-324.e4 (2019). PubMed: 30527663
- D'Lima NG et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 13:174-180 (2017). PubMed: 27918561
