Anti-Patched / PTCH1 antibody (ab51983)
Key features and details
- Goat polyclonal to Patched / PTCH1
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Patched / PTCH1 antibody
See all Patched / PTCH1 primary antibodies -
Description
Goat polyclonal to Patched / PTCH1 -
Host species
Goat -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 161 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 161 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. -
Tissue specificity
In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin. -
Involvement in disease
Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.
Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].
Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
Sequence similarities
Belongs to the patched family.
Contains 1 SSD (sterol-sensing) domain. -
Developmental stage
In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. -
Post-translational
modificationsGlycosylation is necessary for SHH binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5727 Human
- Entrez Gene: 19206 Mouse
- Omim: 601309 Human
- SwissProt: Q13635 Human
- SwissProt: Q86XG7 Human
- SwissProt: Q61115 Mouse
- Unigene: 494538 Human
- Unigene: 228798 Mouse
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Alternative names
- A230106A15Rik antibody
- BCNS antibody
- FLJ26746 antibody
see all
Images
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Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining Patched / PTCH1. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.
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Anti-Patched / PTCH1 antibody (ab51983) at 1 µg/ml + Human Brain lysate in RIPA buffer at 35 µg
Predicted band size: 161 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab51983 has been referenced in 4 publications.
- Hu L et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci 22:N/A (2021). PubMed: 34948290
- Konitsiotis AD et al. Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells. PLoS One 9:e89899 (2014). WB ; Human . PubMed: 24608521
- Kagawa H et al. A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of Mammalian patched 1. PLoS One 6:e18638 (2011). ICC/IF ; Mouse . PubMed: 21533246
- Chang SC et al. Two distinct sites in sonic hedgehog combine for heparan sulfate interactions and cell signaling functions. J Biol Chem 286:44391-402 (2011). WB ; Human . PubMed: 22049079