Anti-PATZ1 antibody (ab154025)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-PATZ1 antibody
See all PATZ1 primary antibodies -
Description
Rabbit polyclonal to PATZ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rabbit, Cow
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Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 249-468 of Human PATZ1 (UniProt: Q9HBE1).
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Positive control
- HepG2, HCT116 cells; A549 and HeLa whole cell lysate (ab150035); HeLa cells; Human N87 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 74 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Involvement in disease
A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 A.T hook DNA-binding domain.
Contains 1 BTB (POZ) domain.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 532416 Cow
- Entrez Gene: 23598 Human
- Entrez Gene: 56218 Mouse
- Entrez Gene: 100328729 Rabbit
- Omim: 605165 Human
- SwissProt: Q9HBE1 Human
- Unigene: 731398 Human
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Alternative names
- 8430401L15Rik antibody
- AI447519 antibody
- and zinc finger-containing protein 1 antibody
see all
Images
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Western blot - Anti-PATZ1 antibody (ab154025)All lanes : Anti-PATZ1 antibody (ab154025) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PATZ1 with ab154025 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)Immunohistochemical analysis of paraffin-embedded Human N87 xenograft tissue labeling PATZ1 with ab154025 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Iesato A et al. PATZ1 knockdown enhances malignant phenotype in thyroid epithelial follicular cells and thyroid cancer cells. Oncotarget 8:82754-82772 (2017). Read more (PubMed: 29137300) »
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). Read more (PubMed: 27391343) »
