Anti-PATZ1 antibody (ab154025)

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Western blot - Anti-PATZ1 antibody (ab154025)
  • Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)

Key features and details

  • Rabbit polyclonal to PATZ1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PATZ1 antibody
    See all PATZ1 primary antibodies

  • Description

    Rabbit polyclonal to PATZ1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 249-468 of Human PATZ1 (UniProt: Q9HBE1).

  • Positive control

    • HepG2, HCT116 cells; A549 and HeLa whole cell lysate (ab150035); HeLa cells; Human N87 xenograft tissue.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rabbit
Cow
Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Transcriptional repressor.

  • Tissue specificity

    Ubiquitous.

  • Involvement in disease

    A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1.

  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 BTB (POZ) domain.
    Contains 7 C2H2-type zinc fingers.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 8430401L15Rik antibody
      • AI447519 antibody
      • and zinc finger-containing protein 1 antibody
      • AT hook- antibody
      • BTB POZ domain zinc finger transcription factor antibody
      • BTB/POZ domain zinc finger transcription factor antibody
      • dJ400N23 antibody
      • Mazr antibody
      • PATZ antibody
      • PATZ1 antibody
      • PATZ1_HUMAN antibody
      • POZ (BTB) and AT hook containing zinc finger 1 antibody
      • POZ , AT hook, and zinc finger containing protein 1 antibody
      • POZ- antibody
      • Protein kinase A RI subunit alpha associated protein antibody
      • Protein kinase A RI subunit alpha-associated protein antibody
      • RIAZ antibody
      • Transcription factor MAZR antibody
      • ZBTB19 antibody
      • Zfp278 antibody
      • Zinc finger and BTB domain containing protein 19 antibody
      • Zinc finger and BTB domain-containing protein 19 antibody
      • Zinc finger protein 278 antibody
      • Zinc finger protein 278, isoform CRA_d antibody
      • Zinc finger sarcoma gene protein antibody
      • ZNF278 antibody
      • ZSG antibody
      see all

    Images

    • Western blot - Anti-PATZ1 antibody (ab154025)
      Western blot - Anti-PATZ1 antibody (ab154025)
      All lanes : Anti-PATZ1 antibody (ab154025) at 1/1000 dilution

      Lane 1 : A549 whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 74 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)
      Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PATZ1 with ab154025 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)
      Immunohistochemical analysis of paraffin-embedded Human N87 xenograft tissue labeling PATZ1 with ab154025 at 1/500 dilution.

    References (3)

    Publishing research using ab154025? Please let us know so that we can cite the reference in this datasheet.

    ab154025 has been referenced in 3 publications.

    • Alda-Catalinas C  et al. A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program. Cell Syst 11:25-41.e9 (2020). PubMed: 32634384
    • Iesato A  et al. PATZ1 knockdown enhances malignant phenotype in thyroid epithelial follicular cells and thyroid cancer cells. Oncotarget 8:82754-82772 (2017). PubMed: 29137300
    • Ho MY  et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). PubMed: 27391343

    Customer reviews and Q&As

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    Western blot abreview for Anti-PATZ1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    150000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

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    Verified customer

    Submitted Nov 06 2013

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