Anti-PATZ1 antibody (ab154025)
Key features and details
- Rabbit polyclonal to PATZ1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PATZ1 antibody
See all PATZ1 primary antibodies -
Description
Rabbit polyclonal to PATZ1 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 249-468 of Human PATZ1 (UniProt: Q9HBE1).
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Positive control
- HepG2, HCT116 cells; A549 and HeLa whole cell lysate (ab150035); HeLa cells; Human N87 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rabbit
Cow
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Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Transcriptional repressor. -
Tissue specificity
Ubiquitous. -
Involvement in disease
A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 1 A.T hook DNA-binding domain.
Contains 1 BTB (POZ) domain.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 532416 Cow
- Entrez Gene: 23598 Human
- Entrez Gene: 56218 Mouse
- Entrez Gene: 100328729 Rabbit
- Omim: 605165 Human
- SwissProt: Q9HBE1 Human
- Unigene: 731398 Human
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Alternative names
- 8430401L15Rik antibody
- AI447519 antibody
- and zinc finger-containing protein 1 antibody
see all
Images
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All lanes : Anti-PATZ1 antibody (ab154025) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PATZ1 with ab154025 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)Immunohistochemical analysis of paraffin-embedded Human N87 xenograft tissue labeling PATZ1 with ab154025 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154025 has been referenced in 3 publications.
- Alda-Catalinas C et al. A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program. Cell Syst 11:25-41.e9 (2020). PubMed: 32634384
- Iesato A et al. PATZ1 knockdown enhances malignant phenotype in thyroid epithelial follicular cells and thyroid cancer cells. Oncotarget 8:82754-82772 (2017). PubMed: 29137300
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). PubMed: 27391343