Overview

  • Product name
    Anti-PATZ1 antibody
    See all PATZ1 primary antibodies
  • Description
    Rabbit polyclonal to PATZ1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 249-468 of Human PATZ1 (UniProt: Q9HBE1).

  • Positive control
    • HepG2, HCT116 cells; A549 and HeLa whole cell lysates; HeLa cells; Human N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Transcriptional repressor.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    A chromosomal aberration involving PATZ1 is associated with small round cell sarcoma. Translocation t(1;22)(p36.1;q12) with EWSR1.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 BTB (POZ) domain.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • 8430401L15Rik antibody
      • AI447519 antibody
      • and zinc finger-containing protein 1 antibody
      • AT hook- antibody
      • BTB POZ domain zinc finger transcription factor antibody
      • BTB/POZ domain zinc finger transcription factor antibody
      • dJ400N23 antibody
      • Mazr antibody
      • PATZ antibody
      • PATZ1 antibody
      • PATZ1_HUMAN antibody
      • POZ (BTB) and AT hook containing zinc finger 1 antibody
      • POZ , AT hook, and zinc finger containing protein 1 antibody
      • POZ- antibody
      • Protein kinase A RI subunit alpha associated protein antibody
      • Protein kinase A RI subunit alpha-associated protein antibody
      • RIAZ antibody
      • Transcription factor MAZR antibody
      • ZBTB19 antibody
      • Zfp278 antibody
      • Zinc finger and BTB domain containing protein 19 antibody
      • Zinc finger and BTB domain-containing protein 19 antibody
      • Zinc finger protein 278 antibody
      • Zinc finger protein 278, isoform CRA_d antibody
      • Zinc finger sarcoma gene protein antibody
      • ZNF278 antibody
      • ZSG antibody
      see all

    Images

    • Western blot - Anti-PATZ1 antibody (ab154025)
      Western blot - Anti-PATZ1 antibody (ab154025)
      All lanes : Anti-PATZ1 antibody (ab154025) at 1/1000 dilution

      Lane 1 : A549 whole cell lysate
      Lane 2 : HeLa whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 74 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)
      Immunocytochemistry/ Immunofluorescence - Anti-PATZ1 antibody (ab154025)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PATZ1 with ab154025 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PATZ1 antibody (ab154025)
      Immunohistochemical analysis of paraffin-embedded Human N87 xenograft tissue labeling PATZ1 with ab154025 at 1/500 dilution.

    References

    This product has been referenced in:
    • Iesato A  et al. PATZ1 knockdown enhances malignant phenotype in thyroid epithelial follicular cells and thyroid cancer cells. Oncotarget 8:82754-82772 (2017). Read more (PubMed: 29137300) »

    See 1 Publication for this product

    Publishing research using ab154025? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-PATZ1 antibody

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    150000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HEK293T cells)
    Specification
    HEK293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Verified customer

    Submitted Nov 06 2013

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