Recombinant Anti-PAX1 antibody [5A2] (ab252847)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [5A2] to PAX1
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-PAX1 antibody [5A2]
See all PAX1 primary antibodies -
Description
Rat monoclonal [5A2] to PAX1 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein (GST-tag) corresponding to Mouse PAX1.
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Positive control
- IHC-P: Mouse embroy day 14.5 tissue. IHC-Fr: Mouse E14.5 embryo tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
5A2 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use a concentration of 28.76 µg/ml.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IHC-P |
Use a concentration of 1.438 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-Fr
Use a concentration of 28.76 µg/ml. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IHC-P
Use a concentration of 1.438 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column. -
Sequence similarities
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 18503 Mouse
- SwissProt: P09084 Mouse
- Unigene: 315502 Mouse
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Alternative names
- HUP48 antibody
- OTTHUMP00000030407 antibody
- Paired box 1 antibody
see all
Images
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling PAX1 with ab252847 at 128.76 µg/mL followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at dilution (Green). Positive staining on mouse embryo is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of paraffin-embedded mouse embroy day 14.5 tissue labeling PAX1 with ab252847 at 1.438 μg/mL followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on the intervertebral disk in mouse embroy day 14.5. The section was incubated with ab252847 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab252847 has been referenced in 3 publications.
- Ren W et al. Cisplatin attenuates taste cell homeostasis and induces inflammatory activation in the circumvallate papilla. Theranostics 13:2896-2913 (2023). PubMed: 37284449
- Arostegui M et al. Cellular taxonomy of Hic1+ mesenchymal progenitor derivatives in the limb: from embryo to adult. Nat Commun 13:4989 (2022). PubMed: 36008423
- Feederle R et al. Generation of Pax1/PAX1-Specific Monoclonal Antibodies. Monoclon Antib Immunodiagn Immunother 35:259-262 (2016). PubMed: 27705080