Overview

  • Product name

  • Description

    Rabbit polyclonal to Pax2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC - Wholemountmore details
  • Species reactivity

    Reacts with: Zebrafish, Medaka fish
  • Immunogen

    Recombinant fragment within Zebrafish Pax2 (internal sequence). The exact sequence is proprietary.
    Database link: Q90268

  • Positive control

    • IHC-Wm: 2 days-post-fertilization zebrafish embryo; 7 days-post-fertilization medaka embryo. WB: 2 days and 36 hour-post-fertilization zebrafish embryo extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 44 kDa.
IHC - Wholemount 1/100 - 1/500.

Target

  • Relevance

    Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localization

    Nuclear
  • Database links

    • Alternative names

      • FSGS7 antibody
      • Paired box 2 antibody
      • Paired box gene 2 antibody
      • paired box homeotic gene 2 antibody
      • paired box protein 2 antibody
      • Paired box protein Pax 2 antibody
      • Paired box protein Pax-2 antibody
      • Paired box protein Pax2 antibody
      • PAPRS antibody
      • Pax 2 antibody
      see all

    Images

    • 2 days-post-fertilization zebrafish embryo stained for Pax2 (green) with ab229318 at a 1/200 dilution in immunohistochemical analysis. Alpha tubulin (acetyl Lys40) (red).

    • 7 days-post-fertilization medaka embryo stained for Pax2a (green) with ab229318 at a 1/100 dilution in immunohistochemical analysis.

    • Anti-Pax2 antibody (ab229318) at 1/1000 dilution + Zebrafish embryo 36 hours post-fertilization extracts at 30 µg

      Developed using the ECL technique.

      Predicted band size: 44 kDa



      10% SDS-PAGE gel.

    • Anti-Pax2 antibody (ab229318) at 1/1000 dilution + Zebrafish embryo 2 days post-fertilization extracts at 30 µg

      Secondary
      HRP-conjugated anti-rabbit IgG

      Developed using the ECL technique.

      Predicted band size: 44 kDa



      10% SDS-PAGE gel.

    • 2 days-post-fertilization zebrafish embryo stained for Pax2a (green) with ab229318 at a 1/200 dilution in immunohistochemical analysis. Alpha tubulin (acetyl Lys40) (red).

    References

    ab229318 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Spotted catshark Tissue sections (Whole embryo section)
    Permeabilization
    Yes - TritonX100 0.25% or Tween20 0.1%
    Specification
    Whole embryo section
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Dr. Rami Reshef

    Verified customer

    Submitted Feb 04 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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