Overview

  • Product name
  • Description
    Rabbit polyclonal to Pax2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Zebrafish
  • Immunogen

    A region within synthetic peptide: DPVGSYSING ILGIPRSNGE KRKRDEDVSE GSVPNGDSQS GVDSLRKHLR, corresponding to internal sequence amino acids 180-229 of Human Pax2

  • Positive control
    • IHC-P: Human kidney. ICC: OVCA429 cells (see review) WB: Human liver tissue, OVCAR-5, OVCAR-3, SKOV3, CaoV3 cell lysates (See review)

Properties

Applications

Our Abpromise guarantee covers the use of ab23799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF 1/250. Fix with 3% paraformaldehyde/2% sucrose (see review by R Drapkin).
WB Use a concentration of 1 - 2.5 µg/ml. Detects a band of approximately 34, 41 kDa (predicted molecular weight: 44 , 42 kDa).Can be blocked with Human Pax2 peptide (ab111670). Dilute antibody in 5% milk in PBS buffer. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

Target

  • Relevance
    Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • FSGS7 antibody
    • Paired box 2 antibody
    • Paired box gene 2 antibody
    • paired box homeotic gene 2 antibody
    • paired box protein 2 antibody
    • Paired box protein Pax 2 antibody
    • Paired box protein Pax-2 antibody
    • Paired box protein Pax2 antibody
    • PAPRS antibody
    • Pax 2 antibody
    see all

Images

  • Anti-Pax2 antibody (ab23799) at 2.5 µg/ml + Fetal kidney lysate

    Predicted band size: 44 , 42 kDa
    Observed band size: 34,41 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab23799 at a concentration of 4-8µg/ml. The arrows indicate the positively labelled cells, epithelial cells of the renal tube.

  • The human ovarian carcinoma cell line OVCA429 was used with ab23799 in ICC/IF. The cells were fixed in paraformaldehyde. Blocking was in 5% serum for 1 hour. The primary antibody was incubated at a dilution of 1/250 for one hour. Immunofluorescence (ICC/IF) only worked with 3% paraformaldehyde/2% sucrose fixation. When 70% methanol/30% acetone fixation was tried non-specific cytoplasmic staining was seen. In the left hand panel staining with DAPI is shown, and in the right staining with the ab23799 antibody.

References

This product has been referenced in:
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab23799 Pax2 antibody has never been tested with samples from chicken. All tested and guaranteed species cross-reactivity and application information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I can confirm that the immunogen for the antibody has 98% alignment with the chicken protein. This is very high, and so it is likely that the antibody will detect in chicken. However, I am sorry we are not able to provide our guarantee for this without laboratory testing.

If you would like to test the antibody in chicken, please do not hesitate to contact me by replying to this message prior to the purchase as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cell)
Permeabilization
Yes - 0,25% Triton X-100
Specification
differentiated induced pluripotent stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 05 2017

Question
Answer

Thank you for your inquiry.

We recommend to use this antibody ata concntration of 4ug/ml in IHC-P (since the conecntration of ab23799 is 1mg/ml, this is a 1:250 dilution). This is only a recommended starting dilution and may well have to be optimized by your customer according to their needs and samples.

If the recommended dilution is used and ca 50ul per slide (this depends on the size of the sample on the slide, it needs to be covered all the way) then one vial of ab23799 will be enough for250 slides.

Our datasheet gives all the information to calculate this. Please follow this link to review the datasheet:

https://www.abcam.com/index.html?datasheet=23799 (or use the following: https://www.abcam.com/index.html?datasheet=23799).

I hope this information was helpful and wish your customer good luck with their research.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Day 14.5 mouse embryo)
Specification
Day 14.5 mouse embryo
Fixative
10% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - 0.1% Triton
Blocking step
Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Apr 02 2012

Question
Answer

DISCOUNT CODE:*********
Expiration date: ********


I am very pleased to hear you would like to accept our offer and test ab23799 in mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your reply.

Unfortunately the number you provided is not an Abcam order/reference number. Did you order the antibody directly from Abcam, or through a sub-dealer?

If you can’t find the original Abcam order number, can you let me know the date it was ordered, the institution it was sent too and who placed the order and I should be able to find your order that way.

I look forward to your reply.

Read More

Question
Answer

Thank you very much for your interest in ab23799.

Aswe talkedabout on the phone ab23799 has not been tested in mouse tissue. Therefore, I can offer a discount off a future purchase if you can provide me with your original Abcam order number for ab23799, test it in mouse tissue and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab23799 in mouse tissue. I will then send a discount code. This code must be issued before purchasing ab23799 so please wait for my reply before ordering.

2. Purchase ab23799 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse tissue.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab23799 turns out to be unsuitable formouse tissue, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your reply.

I am pleased to hear you would be interested in trying ab23799 in chicken samples. I am sorry that because we have over 70 000 products, it is regrettablynot feasible for us to have samples vials to send out to customers.

However, I can offer a discount off a future purchase if you buy ab23799 now, test it inchicken and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab23799 in chicken. I will then send a discount code. This code must be issued before purchasing ab23799 so please wait for my reply before ordering.

2. Purchase ab23799 either by phone, fax, or online (www.abcam.com).

3. Test it in chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab23799 turns out to be unsuitable for chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

Read More

Answer

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody purchased. These conditions will vary among our antibodies, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 6 months from the date of purchase when stored correctly.

For more information on antibody storage and stability, please visit our Antibody Storage Guide on our Protocols and Troubleshooting Tips page (https://www.abcam.com/index.html?pageconfig=resource&rid=10795, and attached).

I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Answer

DISCOUNT CODE: XXXXXXXX Expiration date: 13-01-2012 I am very pleased to hear you would like to accept our offer and test ab23799 in Zebrafish. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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