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    pax2-antibody-ep3251-ab79389.pdf

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RecombinantRabMAb

Recombinant Anti-Pax2 antibody [EP3251] (ab79389)

  • Datasheet
  • SDS
Reviews (11)Q&A (8)References (32)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Western blot - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
  • Anti-Pax2 antibody [EP3251] (ab79389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3251] to Pax2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free HRP PE

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Overview

  • Product name

    Anti-Pax2 antibody [EP3251]
    See all Pax2 primary antibodies
  • Description

    Rabbit monoclonal [EP3251] to Pax2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pax2 aa 1-100. The exact sequence is proprietary.
    Database link: Q02962
    (Peptide available as ab188213)

  • Positive control

    • WB: HEK-293 transfected with his tagged human full-length PAX2. IHC-P: Human, rat and mouse kidney tissues, Human kidney cancer tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.09 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 5.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3251
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Pax2 antibody [EP3251] (ab218058)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunizing Peptide (Blocking)

    • Pax2 peptide (ab188213)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Pax2 protein (ab159050)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (1)
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Pax2 peptide (ab188213).
IHC-P (2)
1/500 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (3)
1/50.
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Pax2 peptide (ab188213).
IHC-P
1/500 - 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
1/50.

Target

  • Relevance

    Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 5076 Human
    • Entrez Gene: 18504 Mouse
    • Entrez Gene: 293992 Rat
    • Omim: 167409 Human
    • SwissProt: Q02962 Human
    • SwissProt: P32114 Mouse
    • Alternative names

      • FSGS7 antibody
      • Paired box 2 antibody
      • Paired box gene 2 antibody
      • paired box homeotic gene 2 antibody
      • paired box protein 2 antibody
      • Paired box protein Pax 2 antibody
      • Paired box protein Pax-2 antibody
      • Paired box protein Pax2 antibody
      • PAPRS antibody
      • Pax 2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument 

    • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)Lee SB et al. PAX2 regulates ADAM10 expression and mediates anchorage-independent cell growth of melanoma cells. PLoS One 6:e22312 (2011). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Pax2 is expressed in melanocytes of benign nevi (A) and melanoma cells of patients with malignant melanoma (B).

      Cells were grown on coverslips and fixed with 4% paraformaldehyde/PBS. After washing the cells with PBS, cells were permeabilized and blocked with 0.1% Triton X-100/PBS containing 5% BSA. Pax2 (green) was examined by immunofluorescent analysis using ab79389 at 1/100 dilution (incubated for 1 hour at room temperature). Following 3 times washing, bound antibodies were deteced by Alexa 488 conjugated goat anti-mouse or Cy3 conjugated goat anti-mouse secondary antibodies.

      Following PBS-washing nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI, Blue) and cells were mounted in Fluoromount-G™ and examined by fluorescence microscopy.

      White arrows in the higher magnified insets indicate PAX2 expression in nucleoli of melanocytes of benign nevi (A), yellow arrows in the higher magnified insets specify PAX2 expression in nucleoli of melanoma cells (B).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)Lee SB et al. PAX2 regulates ADAM10 expression and mediates anchorage-independent cell growth of melanoma cells. PLoS One 6:e22312 (2011).

      Immunohistochemical analysis of Pax2 expression in tissue sections of human benign nevi and malignant melanoma using ab79389 at 1/100 dilution.

      All specimens were fixed in 4% formaline (pH 7.4), embedded in paraffin followed by cutting with a microtome (3 µm thickness). The slides were deparaffinized in xylol for 20 minutes and then rehydrated in descending series of ethanol (100%, 100%, 96%, 96%, 70%, and 70%). For antigen retrieval the slides were boiled in citrate buffer (pH 6.0) for 40 min, and then allowed to cool down for 15 min. After washing with PBS buffer the endogenous peroxidase was blocked with H2O2 for 15 min at room temperature. After washing in PBS the slides were incubated with the antibody against PAX2 (dilution 1∶100) for 60 min at room temperature and washed in PBS again.

      The secondary antibody was incubated for 20 min at room temperature and after washing the slides in PBS the biotin streptavidine label was incubated for 20 min at room temperature. A detection kit including horseradish peroxidase and diaminobenzidine as chromogene was applied for 5 min. Counterstaining was performed with hematoxilin for 6 min.

      (A) In normal sweat glands, Pax2 is expressed in gland epithelial cells (black arrows) while intermingled stromal cells only show very weak or absent nuclear Pax2 expression (green arrow) Bar represents 100 µm.

      (B, C) Normal appearing epidermal cell layers adjacent to (B, bar represent 100 µm) nevi or (C, bar represent 200 µm) malignant melanoma show a differentially Pax2 expression with strongest Pax2 levels in germinal basal cell layers (black arrows) decreasing in higher differentiated keratinocytes and finally being absent in corneocytes (green arrows).

      (D) Malignant melanoma cells - heterogeneous nuclear Pax2 expression. Strongest expression in large atypical nuclei with prominent nucleoli (black arrows). 

      (E, F) Pax2 expression in intradermal nevi was heterogeneous and did not correlate with histological features.

    • Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (ab79389)
      Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (ab79389)

      Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast)labelling Pax2 with ab79389 at 1/30. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)Image from Lee SB et al., PLoS One. 2011;6(8):e22312. Epub 2011 Aug 18. Fig 5.; doi:10.1371/journal.pone.0022312; August 18, 2011, PLoS ONE 6(8): e22312.

      Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with unpurified ab79389.
      Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3®-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    • Western blot - Anti-Pax2 antibody [EP3251] (ab79389)
      Western blot - Anti-Pax2 antibody [EP3251] (ab79389)
      All lanes : Anti-Pax2 antibody [EP3251] (ab79389) at 1/10000 dilution

      Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) transfected with empty vector whole cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) transfected with his tagged human full-length PAX2

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 45 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument 

    • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labelling Pax2 with ab79389 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. . Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue). Confocal image showing nucleolar staining in HepG2 cell line.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (A) human fetal kidney, (B) human normal kidney and (C) human renal cell carcinoma tissues labelled Pax2 with unpurified ab79389 at a dilution of 1/1000.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument 

    • Anti-Pax2 antibody [EP3251] (ab79389)
      Anti-Pax2 antibody [EP3251] (ab79389)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (32)

    Publishing research using ab79389? Please let us know so that we can cite the reference in this datasheet.

    ab79389 has been referenced in 32 publications.

    • Petrovic A  et al. Establishment of Long-Term Primary Cortical Neuronal Cultures From Neonatal Opossum Monodelphis domestica. Front Cell Neurosci 15:661492 (2021). PubMed: 33815068
    • Vitkunaite A  et al. Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy. Sci Rep 11:6275 (2021). PubMed: 33737593
    • Lowenstein ED  et al. Olig3 regulates early cerebellar development. Elife 10:N/A (2021). PubMed: 33591268
    • Sun X  et al. ADNP promotes neural differentiation by modulating Wnt/ß-catenin signaling. Nat Commun 11:2984 (2020). PubMed: 32533114
    • Strickland AL  et al. PI3K Pathway Effectors pAKT and FOXO1 as Novel Markers of Endometrioid Intraepithelial Neoplasia. Int J Gynecol Pathol 38:503-513 (2019). PubMed: 30256235
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 19 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pax2 antibody [EP3251]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HAFs)
    Permeabilization
    Yes - 0.2% TritonX100/PBS
    Specification
    HAFs
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 18 2022

    IHC - Wholemount abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Embryo (spinal cord)
    Specification
    spinal cord
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Nov 23 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (human embryo urogenital ducts)
    Permeabilization
    Yes - Triton
    Specification
    human embryo urogenital ducts
    Blocking step
    Gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Alain Chedotal

    Verified customer

    Submitted Nov 23 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Pax2 antibody [EP3251]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (Optic nerve)
    Permeabilization
    Yes - TritonX-100 + Tween20
    Specification
    Optic nerve
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Optic nerve)
    Permeabilization
    Yes - TritonX-100 + Tween20
    Specification
    Optic nerve
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pax2 antibody [EP3251]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monodelphis domestica Cell (Cortex)
    Permeabilization
    Yes - 0,1% Triton X-100 in PBS
    Specification
    Cortex
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Jelena Ban

    Verified customer

    Submitted May 29 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Cat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Tween20
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 06 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer (PH6)
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    x0909 (DAKO blocking product) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 29 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pax2 antibody [EP3251]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Human Cell (differentiated iPSC)
    Specification
    differentiated iPSC
    Permeabilization
    Yes - Triton X
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 26 2015

    Question

    I would like to inquire about the initial concnetration of Pax2
    primary antibody[EP3251].(ab79389) to be used in IHC for antibody
    titration needed for the negative control

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2012

    Answer

    Thank you again for your email.
    The concentration of your lot is 0.2290 mg/ml.

    Please let me know if you have anymore questions.

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2012

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