Overview

  • Product name

    Anti-Pax2 antibody [EPR8586]
    See all Pax2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8586] to Pax2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Pax2 aa 150-350.

  • Positive control

    • Human fetal kidney lysate. Human clear renal carcinoma tissue and Human fetal kidney tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 o C, below boiling, and then let cool for 10-20 min.
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Relevance

      Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • FSGS7 antibody
      • Paired box 2 antibody
      • Paired box gene 2 antibody
      • paired box homeotic gene 2 antibody
      • paired box protein 2 antibody
      • Paired box protein Pax 2 antibody
      • Paired box protein Pax-2 antibody
      • Paired box protein Pax2 antibody
      • PAPRS antibody
      • Pax 2 antibody
      see all

    Images

    • Anti-Pax2 antibody [EPR8586] (ab150391) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Pax2 with ab150391 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human clear renal carcinoma tissue labelling Pax2 with ab150391 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human Wilms Tumor tissue using ab150391 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150391 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150391 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150391 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab150391 showing -ve staining.

    References

    ab150391 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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