Name: Anti-Pax2 antibody [EPR8586]
Brand: RabMAb
SKU: ab150391
Rating: 5 (1 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8586] to Pax2
Suitable for: IHC-P, WB
Reacts with: Human

Carrier Free

Product name

Anti-Pax2 antibody [EPR8586]
See all Pax2 primary antibodies
Description

Rabbit monoclonal [EPR8586] to Pax2
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Recombinant fragment corresponding to Human Pax2 aa 150-350.
Positive control
- Human fetal kidney lysate. Human clear renal carcinoma tissue and Human fetal kidney tissue.
General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8586
Isotype

IgG
Research areas

Alternative Versions
- Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Pax2 protein (ab159050)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(1)	1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 ^o C, below boiling, and then let cool for 10-20 min.
WB		1/1000 - 1/10000. Predicted molecular weight: 45 kDa.

Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 ^o C, below boiling, and then let cool for 10-20 min.
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.

Relevance

Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.
Cellular localization

Nuclear
Database links
- Entrez Gene: 5076 Human
- Omim: 167409 Human
- SwissProt: Q02962 Human
Alternative names
- FSGS7 antibody
- Paired box 2 antibody
- Paired box gene 2 antibody
- paired box homeotic gene 2 antibody
- paired box protein 2 antibody
- Paired box protein Pax 2 antibody
- Paired box protein Pax-2 antibody
- Paired box protein Pax2 antibody
- PAPRS antibody
- Pax 2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Pax2 with Purified ab150391 at 1:250 dilution (5.35 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Pax2 antibody [EPR8586] (ab150391)

All lanes : Anti-Pax2 antibody [EPR8586] (ab150391) at 1/10000 dilution

Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : 293T transfected with Pax2 expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Pax2 with ab150391 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin-embedded Human clear renal carcinoma tissue labelling Pax2 with ab150391 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin embedded Human Wilms Tumor tissue using ab150391 at 1/250 dilution showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150391 at 1/250 dilution showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Pax2 antibody [EPR8586] (ab150391)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab150391? Please let us know so that we can cite the reference in this datasheet.

ab150391 has been referenced in 3 publications.

Mayer C et al. Histomorphology and Immunohistochemistry of a Congenital Nephromegaly Demonstrate Concurrent Features of Heritable and Acquired Cystic Nephropathies in a Girgentana Goat (Capra falconeri). Case Rep Vet Med 2021:8749158 (2021). PubMed: 33532110
Feng Y et al. PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming. Int J Oncol 56:697-708 (2020). PubMed: 31922217
Tate K et al. Endometrial Carcinoma With an Unusual Morphology in a Patient With Cornelia de Lange Syndrome: A Case Study. Int J Gynecol Pathol 38:340-345 (2019). PubMed: 29620584

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Atypical Endometrial Hyperplasia)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Leica Bond Epitope Retreival 2

Specification

Atypical Endometrial Hyperplasia

Blocking step

Leica Bond Polymer Refine Assay as blocking agent for 8 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Sep 06 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pax2 antibody [EPR8586]