Recombinant Anti-Pax2 antibody [EPR8586] (ab150391)
![Western blot - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157006-anti-pax2-antibody-epr8586-western-blot.jpg "Western blot - Anti-Pax2 antibody [EPR8586] (ab150391)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8586] to Pax2
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-Pax2 antibody [EPR8586]
See all Pax2 primary antibodies -
Description
Rabbit monoclonal [EPR8586] to Pax2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Pax2 aa 150-350.
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Positive control
- Human fetal kidney lysate. Human clear renal carcinoma tissue and Human fetal kidney tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8586 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 o C, below boiling, and then let cool for 10-20 min.
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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| Notes |
|---|
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 o C, below boiling, and then let cool for 10-20 min. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
Target
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Relevance
Pax2 is a transcription factor critically required during the development of the nervous and excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract. Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 5076 Human
- Omim: 167409 Human
- SwissProt: Q02962 Human
- Unigene: 155644 Human
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Alternative names
- FSGS7 antibody
- Paired box 2 antibody
- Paired box gene 2 antibody
see all
Images
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Western blot - Anti-Pax2 antibody [EPR8586] (ab150391)Anti-Pax2 antibody [EPR8586] (ab150391) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Secondary
HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157005-anti-pax2-antibody-epr8586-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Pax2 with ab150391 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157004-anti-pax2-antibody-epr8586-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin-embedded Human clear renal carcinoma tissue labelling Pax2 with ab150391 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157003-anti-pax2-antibody-epr8586-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin embedded Human Wilms Tumor tissue using ab150391 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157002-anti-pax2-antibody-epr8586-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150391 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157001-anti-pax2-antibody-epr8586-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150391 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-157000-anti-pax2-antibody-epr8586-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150391 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-156999-anti-pax2-antibody-epr8586-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (ab150391)Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab150391 showing -ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Pax2 antibody [EPR8586] (ab150391)](https://www.abcam.com/ps/products/150/ab150391/Images/ab150391-9-benefits-of-recombinant-antibodies.png)
Anti-Pax2 antibody [EPR8586] (ab150391)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab150391 has been referenced in 2 publications.
- Feng Y et al. PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming. Int J Oncol 56:697-708 (2020). PubMed: 31922217
- Tate K et al. Endometrial Carcinoma With an Unusual Morphology in a Patient With Cornelia de Lange Syndrome: A Case Study. Int J Gynecol Pathol 38:340-345 (2019). PubMed: 29620584