Anti-PAX3 antibody (ab180754)
Key features and details
- Rabbit polyclonal to PAX3
- Suitable for: ICC/IF, ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PAX3 antibody
See all PAX3 primary antibodies -
Description
Rabbit polyclonal to PAX3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis -
Immunogen
Recombinant fragment within Human PAX3 aa 300 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P23760 -
Positive control
- WB: U-251 MG whole cell lysate; ICC: MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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ICC |
1/50 - 1/200.
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IHC-P |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 23 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
ICC
1/50 - 1/200. |
IHC-P
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 23 kDa. |
Target
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Function
Probable transcription factor associated with development of alveolar rhabdomyosarcoma. -
Involvement in disease
Defects in PAX3 are the cause of Waardenburg syndrome type 1 (WS1) [MIM:193500]. WS1 is an autosomal dominant disorder characterized by wide bridge of nose owing to lateral displacement of the inner canthus of each eye (dystopia canthorum), pigmentary disturbances such as frontal white blaze of hair, heterochromia of irides, white eyelashes, leukoderma and sensorineural deafness. The syndrome shows variable clinical expression and some affected individuals do not manifest hearing impairment.
Defects in PAX3 are the cause of Waardenburg syndrome type 3 (WS3) [MIM:148820]; also known as Klein-Waardenburg syndrome or Waardenburg syndrome with upper limb anomalies or white forelock with malformations. WS3 is a very rare autosomal dominant disorder, which shares many of the characteristics of WS1. Patients additionally present with musculoskeletal abnormalities.
Defects in PAX3 are the cause of craniofacial-deafness-hand syndrome (CDHS) [MIM:122880]. CDHS is thought to be an autosomal dominant disease which comprises absence or hypoplasia of the nasal bones, hypoplastic maxilla, small and short nose with thin nares, limited movement of the wrist, short palpebral fissures, ulnar deviation of the fingers, hypertelorism and profound sensory-neural deafness.
Defects in PAX3 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX3 is found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with FOXO1. The resulting protein is a transcriptional activator.
Note=A chromosomal aberration involving PAX3 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with NCOA1 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5077 Human
- Entrez Gene: 18505 Mouse
- Entrez Gene: 114502 Rat
- Omim: 606597 Human
- SwissProt: P23760 Human
- SwissProt: P24610 Mouse
- Unigene: 42146 Human
- Unigene: 1371 Mouse
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Alternative names
- CDHS antibody
- HUP 2 antibody
- HUP2 antibody
see all
Images
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All lanes : Anti-PAX3 antibody (ab180754) at 1/1000 dilution
Lane 1 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) (HRP) at 1/10000 dilution
Predicted band size: 23 kDaBlocking buffer: 3% NFDM/TBST.
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Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180754. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab180754 has been referenced in 11 publications.
- Guo D et al. iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling. Elife 11:N/A (2022). PubMed: 35076017
- Grunow B et al. Histological and biochemical evaluation of skeletal muscle in the two salmonid species Coregonus maraena and Oncorhynchus mykiss. PLoS One 16:e0255062 (2021). PubMed: 34383783
- Zhu X et al. Circular RNA circANKIB1 promotes the progression of osteosarcoma by regulating miR-217/PAX3 axis. J Bone Oncol 27:100347 (2021). PubMed: 33552886
- Lv WL et al. PAX3 silencing suppresses gastric cancer proliferation and angiogenesis via MET/PI3K signaling. Neoplasma 67:304-311 (2020). PubMed: 31847528
- Zhang H et al. miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. Redox Biol 29:101402 (2020). PubMed: 31926626