Overview

  • Product name
  • Description
    Rabbit polyclonal to PAX4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: RTIFSPSQAE ALEKEFQRGQ YPDSVARGKL ATATSLPEDT VRVWFSNRRA , corresponding to Internal sequence amino acids 184-233 of Human PAX4

  • Positive control
    • Jurkat cell lysate; Human intestinal tissue; Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab42450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/312500.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
  • Involvement in disease
    Defects in PAX4 are a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2 or maturity-onset diabetes. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Genetic variations in PAX4 are associated with susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100]. IDDM normally starts in childhood or adolescence and is caused by the body's own immune system which destroys the insulin-producing beta cells in the pancreas. Classical features are polydipsia, polyphagia and polyuria, due to hyperglycemia-induced osmotic diuresis.
    Defects in PAX4 are a cause of susceptibility to diabetes mellitus ketosis-prone (KPD) [MIM:612227]. KPD is an atypical form of diabetes mellitus characterized by an acute initial presentation with severe hyperglycemia and ketosis, as seen in classic type 1 diabetes, but after initiation of insulin therapy, prolonged remission is often possible with cessation of insulin therapy and maintenance of appropriate metabolic control. Metabolic studies show a markedly blunted insulin secretory response to glucose, partially reversible with the improvement of blood glucose control. Variable levels of insulin resistance are observed, especially in obese patients. Pancreatic beta-cell autoimmunity is a rare finding.
    Defects in PAX4 are the cause of maturity-onset diabetes of the young type 9 (MODY9) [MIM:612225]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPD antibody
    • MGC129960 antibody
    • MODY9 antibody
    • Paired box 4 antibody
    • Paired box gene 4 antibody
    • paired box homeotic gene 4 antibody
    • Paired box protein Pax-4 antibody
    • Paired domain gene 4 antibody
    • Pax4 antibody
    • PAX4_HUMAN antibody
    see all

Images

  • Ab42450 at 4µg/ml staining human PAX4 in human epithelial cells of intestinal villus by immunohistochemistry, parafin embedded human intestine tissue.
  • Ab42450 at 4µg/ml staining human PAX4 in epithelial cells of renal tubule by immunohistochemistry, parafin embedded human kidney tissue.
  • Anti-PAX4 antibody (ab42450) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 58 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12% Tris-glycine

References

This product has been referenced in:
  • Zhang L  et al. KIT is an Independent Prognostic Marker for Pancreatic Endocrine Tumors: A Finding Derived From Analysis of Islet Cell Differentiation Markers. Am J Surg Pathol : (2009). IHC-P ; Human . Read more (PubMed: 19574886) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Question
Answer

Thank you for your reply and for providing the order number.


Your credit note ID is *.


I am sorry that these antibodiesdid not perform as stated on the datasheets. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Question
Answer

Thank you for your reply.

I would be happy to process a refund for you, since these antibodies are covered under our Abpromise, which is good for 6 months after purchase. To be able to process the refund, could you please provide me with the either the Abcam order number, or PO# that was used to purchase these antibodies.

I look forward to your reply and resolving this issue for you.

Read More

Answer

Thank you for your reply.

What I meant by different targets against the same antibody, is that we sometimes have multiply antibodies that recognize the same target in our catalogue.

However, of the 3 antibodies you are using, the only other one that we have in the catalogue that we have tested in IHC-P on human tissue is:

https://www.abcam.com/Neurogenin3-antibody-ab38548.html



If you would prefer me to send different antibodies against different targets instead, then I happy to do that. Just let me know the catalogue numbers of the antibodies that you are interested in. Or if you would prefer a refund, I can also do that instead.

Please let me know how you would like to continue.

I look forward to your reply.

Read More

Question

Thanks a lot for your email andresponding to my questions.



Iincludedmore detailsof our IHC-P conditions for the two antibodies. Alltestswere runwithLeica Bond Maxauto-stainer, the sectionthicknessis 4um.



1.Ab71559Hes 1 (Lot #9607-5), tested IHC-P onFFPE human colon tissue (normal and cancer). Antibody isaliquoted into small volume and stored at -20C.



Order information:
HIER:Leica Bond ER1(30minand40min )

note: Leica Bond ER 1 contains citrate based buffer and surfactant, pH5.9-6.1 at 25C, cat# AR9961)



Antibody dilution: 1:100, 1:200 and 1:400. Antibody isdiluted withDako antibody diluent(Dako cat#S0809).



Problem: Observe strongcytoplasmic stainingland cells. No nuclear stain atallat high or low concentration.



Question: We notice there are a fewHes 1 antibody available from abcam. Wonderingif any of them may be applicable on IHC-P, which oneyouwill recommend for us to test. Please all give us suggestion about the tissue type to test.



2.Ab42450 Pax 4 (Lot # GR75087), tested IHC-P onFFPE human colon tissue(normal and cancer). Antibody isaliquoted into small volume and stored at -20C.

HIER:Leica Bond ER1(20min,30minand40min )

Leica Bond ER2(20minand30min )


(note: Leica Bond ER2 contains an EDTA based buffer and surfactant, pH8.9-9.1 at 25C, cat#9640)




Antibody dilution: 1:250, 1:100, 1:200, 1:300and 1:400. Antibody isdiluted withDako antibody diluent(Dako cat#S0809).



Problem: Observe strongcytoplasmic stainingland cells,similar stain pattern asHes1No nuclear stain noticed.



Question: Can we get refund for this one since there is no other pax 4 antibody available from abcam?



Again, thanks for your help and looking forward to your feedback.

Read More
Answer

Thank you for your reply.

In regards to ab71559, although the 6 month gurantee window has passed, I will make an exception and send a free of charge replacement. Below are the links to a few other HEs1 antibodies that are covered under our Abpromise to work in IHC-P on human samples:

https://www.abcam.com/Hes1-antibody-EPR4226-ab108937.html
https://www.abcam.com/Hes1-antibody-2D2-ab119776.html



Please let me know which antibody you would like to receive and I will be happy to send it out.

For ab42450, as requested, I have refunded the cost of the antibody:

Your credit note ID is *****.

If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for your reply and for providing the protocol information.

Based on what you have described, I think that the best way forward in this issue is send free of charge replacements for the antibodies in question. I could either replace the antibodies with a new vial of the same antibody, or replace them with different antibodies against the same target.

Please let me know how you would like to continue and I will be happy to get those replacements to you as quickly as possible.

I look forward to your reply.

Read More

Question
Answer

Thank you for contacting Abcam.

I am sorry about the issues you have been having with both ab71559 and ab42450 in IHC-P. These antibodies are covered under our Abpromise for six months and are guaranteed to work in IHC-P on human samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

I was wondering if you would be able to provide the name of the antigen retrieval solution you are using from Leica, so that I can look into this a little more. As after talking to my colleagues, depending on the buffer you are using, a blocking step may be required. Also how thick are your samples that you are using?

I look forward to your reply and helping resolve this issue.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that you are having some technical issues with a few of our antibodies. These antibodiesare covered under our Abpromise for six months andareguaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

To be able to help you further in this matter, would you please be able to answer a few more questions about the protocol that you are using:

1) Fixation step

Fixation time

Fixation temperature

2) Antigen retrieval method (time and procedure)

3) Permeabilization method:

Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?

Permeabilizing agent and concentration:

4) Blocking agent (eg BSA, serum…):

Concentration

Blocking time

Blocking temperature

5) Endogenous peroxidases blocked?

Endogenous biotins blocked?

6) Primary antibody::





Incubation time

7) Washing after primary and secondary antibodies:

Buffer

Number of washes

8) Detection method

9) How many times have you run this staining?

Do you obtain the same results every time?

I look forward to your reply and helping to resolve this issue.

Read More

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