Recombinant Anti-PAX5 antibody [1H9] (ab211293)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1H9] to PAX5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PAX5 antibody [1H9]
See all PAX5 primary antibodies -
Description
Rat monoclonal [1H9] to PAX5 -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein. This information is considered to be commercially sensitive.
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Positive control
- IHC-P: Human tonsil and mouse spleen tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Aqueous buffer -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
1H9 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab211293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 0.365 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). | |
IHC-P | Use a concentration of 0.73 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. -
Involvement in disease
A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Leukemia, acute lymphoblastic, 3 -
Sequence similarities
Contains 1 paired domain. -
Developmental stage
Expressed at early B-cell differentiation, in the developing CNS and in adult testis. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5079 Human
- Entrez Gene: 18507 Mouse
- Omim: 167414 Human
- SwissProt: Q02548 Human
- SwissProt: Q02650 Mouse
- Unigene: 654464 Human
- Unigene: 439659 Mouse
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Alternative names
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [1H9] (ab211293)
IHC image of PAX5 staining in a section of formalin-fixed paraffin-embedded normal mouse spleen performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab211293, 0.73µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [1H9] (ab211293)
IHC image of PAX5 staining in a section of formalin-fixed paraffin-embedded normal human TONSIL* performed on a Leica BOND™ system using the standard Protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab211293, 0.73 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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All lanes : Anti-PAX5 antibody [1H9] (ab211293) at 1 µg/ml
Lane 1 : Human tonsil whole tissue lysate
Lane 2 : Ramos whole cell lysate
Lane 3 : Daudi whole cell lysate
Lane 4 : Mouse spleen whole tissue lysate
Lane 5 : Rat spleen whole tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-20% Criterion TGX gel in 1X SDS buffer. The gel was run at 180V for 40 minutes before being transferred onto a Nitrocellulose membrane at 25V for 7 minutes using the Trans-Blot turbo transfer system. The membrane was then blocked for an hour using 3% milk before being incubated with ab211293 overnight at 4°C at 1µg/ml. Antibody binding was detected using a Goat anti-Rat antibody conjugated to HRP, and visualised using Bio-Rad Clarity Western ECL substrate.
Protocols
Datasheets and documents
References (1)
ab211293 has been referenced in 1 publication.
- Kallies A et al. Initiation of plasma-cell differentiation is independent of the transcription factor Blimp-1. Immunity 26:555-66 (2007). WB . PubMed: 17509907