Overview

  • Product name

  • Description

    Rabbit polyclonal to PAX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human PAX5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02548

  • Positive control

    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab15164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
IP Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IHC-FoFr 1/50.

Target

  • Function

    May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
  • Involvement in disease

    A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
    Leukemia, acute lymphoblastic, 3
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell lineage specific activator antibody
    • B cell lineage specific activator protein antibody
    • B cell specific activator protein antibody
    • B cell specific transcription factor antibody
    • B-cell-specific transcription factor antibody
    • BSAP antibody
    • EBB-1 antibody
    • KLP antibody
    • Paired box 5 antibody
    • Paired box gene 5 (B cell lineage specific activator protein) antibody
    • Paired box gene 5 (B cell lineage specific activator) antibody
    • Paired box gene 5 antibody
    • Paired box homeotic gene 5 antibody
    • Paired box protein Pax 5 antibody
    • Paired box protein Pax-5 antibody
    • Paired domain gene 5 antibody
    • PAX 5 antibody
    • PAX5 antibody
    • PAX5_HUMAN antibody
    • Transcription factor PAX 5 antibody
    see all

Images

  • PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 42kDa; PAX5

  • ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).

References

This product has been referenced in:

  • Furukawa S  et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017). IHC ; Dog . Read more (PubMed: 28190929) »
  • Perearnau A  et al. p27Kip1, PCAF and PAX5 cooperate in the transcriptional regulation of specific target genes. Nucleic Acids Res 45:5086-5099 (2017). Read more (PubMed: 28158851) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Vielen Dank für Ihren Anruf gestern.

Ich habe für Sie unsere anti Pax5 Antikörper durchgeschaut und muss Ihnen leider mitteilen, dass auch alle polyklonalen mittels Immunogen-Peptiden hergestellt wurden. Diese werden demnach höchstwahrscheinlich auch nur einmal pro Zielprotein binden und keine Amplifikation liefern.

Meines Wissens ist PE ein sehr "helles" Fluorochrom und sollte zum Sortieren gut geeignet sein, obwohl es schnell ausbleicht.

Da Sie Zielproteine im Kern färben, könnte die schwache Färbung daran liegen, dass der sekundäre nicht optimal bindet, da einfach nicht genug Platz ist. Vielleicht kann man das durch Antikörper- Fragmente wie F(ab)2 verbessern.

Bei anti Mum1 Antikörpern haben wir einen, der mit einem Proteinfragment hergestellt worden ist. Aber dass dieser Antikörper an multiplen Stellen bindet und somit das Signal amplifiziert kann ich Ihnen nicht garantieren, da es ja oft ein dominantes Epitop gibt:

ab78312 (Anti-MUM1 antibody)

https://www.abcam.com/index.html?datasheet=78312 (or use the following: https://www.abcam.com/index.html?datasheet=78312).

Es tut mir leid, dass ich keine positiven Nachrichten für Sie habe und hoffe, diese Information ist dennoch hilfreich.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (hematopoietic human cell lines)
Loading amount
10 µg
Specification
hematopoietic human cell lines
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Grazia Fazio

Verified customer

Submitted Jul 23 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse pre-B lymphocytes)
Loading amount
5 µg
Specification
mouse pre-B lymphocytes
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Grazia Fazio

Verified customer

Submitted Jul 23 2010

Answer

I can confirm that the immunogen sequence is within this amino acid segment and therefore the antibody will recognise your fragment,

Read More

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