Key features and details
- Rabbit polyclonal to PAX5
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PAX5 antibody
See all PAX5 primary antibodies
DescriptionRabbit polyclonal to PAX5
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human PAX5 (internal sequence). Expressed in E. coli.
Database link: Q02548
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.01% BSA, 30% Glycerol (glycerin, glycerine), 69% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab183575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 42 kDa.|
|ICC/IF||1/50 - 1/200.|
|IP||Use at 2-5 µg/mg of lysate.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in diseaseA chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Leukemia, acute lymphoblastic, 3
Sequence similaritiesContains 1 paired domain.
Developmental stageExpressed at early B-cell differentiation, in the developing CNS and in adult testis.
- Information by UniProt
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
All lanes : Anti-PAX5 antibody (ab183575) at 1/1000 dilution
Lane 1 : Ramos whole cell lysate
Lane 2 : Daudi whole cell lysate
Lane 3 : Raji whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A20 whole cell lysate
Lysates/proteins at 75 µg per lane.
All lanes : Goat anti rabbit HRP at 1/500 dilution
Predicted band size: 42 kDa
Immunofluorescent analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA for 15 minutes at room temperature. Cells were probed with ab183575 at a dilution of 1/100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
Immunoprecipitation of PAX5 was performed using Raji whole cell lysates. Antigen-antibody complexes were formed by incubating 75 ug of lysate with 5ug of ab183575 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab183575 at a dilution of 1/1000 overnight rotating at 4°C. Membranes were washed in TBST, and probed with an IP Detection Reagent at a dilution of 1/1000 for at least one hour. Chemiluminescent detection was performed.
Flow cytometry analysis of PAX5 (red histogram) on Ramos cells. Cells were harvested, fixed with 4% formaldehyde, permeabilized, washed with PBS, and incubated with ab183575 at a 1/50 dilution or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 488 goat anti-rabbit IgG secondary antibody was performed. 30,000 cells were acquired for analysis.
ab183575 has been referenced in 2 publications.
- Okuyama K et al. PAX5 is part of a functional transcription factor network targeted in lymphoid leukemia. PLoS Genet 15:e1008280 (2019). PubMed: 31381561
- Ott CJ et al. Enhancer Architecture and Essential Core Regulatory Circuitry of Chronic Lymphocytic Leukemia. Cancer Cell 34:982-995.e7 (2018). PubMed: 30503705