Anti-PAX5 antibody (ab183575)

Rabbit polyclonal PAX5 antibody. Validated in WB, IP, ICC, Flow Cyt, ICC/IF and tested in Mouse, Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

  • Description

    Rabbit polyclonal to PAX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PAX5 (internal sequence). Expressed in E. coli.
    Database link: Q02548

  • Positive control

    • Ramos, Daudi, Raji and A20 cell lysate. Ramos cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab183575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.
ICC 1/50 - 1/200.
ICC/IF 1/50 - 1/200.
IP Use at 2-5 µg/mg of lysate.
Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
  • Involvement in disease

    A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
    Leukemia, acute lymphoblastic, 3
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell lineage specific activator antibody
    • B cell lineage specific activator protein antibody
    • B cell specific activator protein antibody
    • B cell specific transcription factor antibody
    • B-cell-specific transcription factor antibody
    • BSAP antibody
    • EBB-1 antibody
    • KLP antibody
    • Paired box 5 antibody
    • Paired box gene 5 (B cell lineage specific activator protein) antibody
    • Paired box gene 5 (B cell lineage specific activator) antibody
    • Paired box gene 5 antibody
    • Paired box homeotic gene 5 antibody
    • Paired box protein Pax 5 antibody
    • Paired box protein Pax-5 antibody
    • Paired domain gene 5 antibody
    • PAX 5 antibody
    • PAX5 antibody
    • PAX5_HUMAN antibody
    • Transcription factor PAX 5 antibody
    see all

Images

  • All lanes : Anti-PAX5 antibody (ab183575) at 1/1000 dilution

    Lane 1 : Ramos whole cell lysate
    Lane 2 : Daudi whole cell lysate
    Lane 3 : Raji whole cell lysate
    Lane 4 : Jurkat whole cell lysate
    Lane 5 : A20 whole cell lysate

    Lysates/proteins at 75 µg per lane.

    Secondary
    All lanes : Goat anti rabbit HRP at 1/500 dilution

    Predicted band size: 42 kDa

  • Immunofluorescent analysis of PAX5 (green) in Ramos and negative control Jurkat cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA for 15 minutes at room temperature. Cells were probed with ab183575 at a dilution of 1/100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1/200 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

  • Immunoprecipitation of PAX5 was performed using Raji whole cell lysates. Antigen-antibody complexes were formed by incubating 75 ug of lysate with 5ug of ab183575 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab183575 at a dilution of 1/1000 overnight rotating at 4°C. Membranes were washed in TBST, and probed with an IP Detection Reagent at a dilution of 1/1000 for at least one hour. Chemiluminescent detection was performed.

  • Flow cytometry analysis of PAX5 (red histogram) on Ramos cells. Cells were harvested, fixed with 4% formaldehyde, permeabilized, washed with PBS, and incubated with ab183575 at a 1/50 dilution or with PBS alone (black histogram) for 1 hour at room temperature. For flow cytometry analysis, 30-minute incubation with DyLight 488 goat anti-rabbit IgG secondary antibody was performed. 30,000 cells were acquired for analysis.

References

ab183575 has not yet been referenced specifically in any publications.

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