Key features and details
- Mouse monoclonal [PCRP-PAX5-1B7] to PAX5
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PAX5 antibody [PCRP-PAX5-1B7]
See all PAX5 primary antibodies
DescriptionMouse monoclonal [PCRP-PAX5-1B7] to PAX5
Tested applicationsSuitable for: WB, IHC-P, Protein Arraymore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PAX5.
Database link: Q02548
- WB: Raji cell lysate. IHC-P: Human lymph node tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from Bioreactor Concentrate by Protein A/G.
Our Abpromise guarantee covers the use of ab236537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 42 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature.
|Protein Array||Use at an assay dependent concentration.|
FunctionMay play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in diseaseA chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Leukemia, acute lymphoblastic, 3
Sequence similaritiesContains 1 paired domain.
Developmental stageExpressed at early B-cell differentiation, in the developing CNS and in adult testis.
- Information by UniProt
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
ab236537 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Formalin-fixed, paraffin-embedded human lymph node tissue stained for PAX5 using ab236537 at 1 μg/ml in immunohistochemical analysis.
Anti-PAX5 antibody [PCRP-PAX5-1B7] (ab236537) at 2 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236537 has not yet been referenced specifically in any publications.