Overview

  • Product name

    Anti-PAX5 antibody [SP34]
    See all PAX5 primary antibodies
  • Description

    Rabbit monoclonal [SP34] to PAX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PAX5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02548

  • Positive control

    • FC: Ramos cells. IHC-P: Human tonsil and B cell lymphoma and rat spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
  • Involvement in disease

    A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
    Leukemia, acute lymphoblastic, 3
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell lineage specific activator antibody
    • B cell lineage specific activator protein antibody
    • B cell specific activator protein antibody
    • B cell specific transcription factor antibody
    • B-cell-specific transcription factor antibody
    • BSAP antibody
    • EBB-1 antibody
    • KLP antibody
    • Paired box 5 antibody
    • Paired box gene 5 (B cell lineage specific activator protein) antibody
    • Paired box gene 5 (B cell lineage specific activator) antibody
    • Paired box gene 5 antibody
    • Paired box homeotic gene 5 antibody
    • Paired box protein Pax 5 antibody
    • Paired box protein Pax-5 antibody
    • Paired domain gene 5 antibody
    • PAX 5 antibody
    • PAX5 antibody
    • PAX5_HUMAN antibody
    • Transcription factor PAX 5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PAX5 with ab227635 at 1/50 dilution (3.44 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228136 for 30 mins at room temperature.

  • Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling PAX5 with purified ab227635 at 1:20 dilution (8.6 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PAX5 with ab227635 at 1/50 dilution (3.44 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228136 for 30 mins at room temperature.

  • Immunohistochemical analysis of Human tonsil tissue labeling PAX5 using ab227635.

  • Immunohistochemical analysis of Human B cell lymphoma tissue staining PAX5 using ab227635. 

References

This product has been referenced in:

  • Liu PJ  et al. In-depth proteomic analysis of six types of exudative pleural effusions for nonsmall cell lung cancer biomarker discovery. Mol Cell Proteomics 14:917-32 (2015). Read more (PubMed: 25638566) »
See 1 Publication for this product

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