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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-PAX6 antibody (ab154253)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
  • Western blot - Anti-PAX6 antibody (ab154253)
  • Immunoprecipitation - Anti-PAX6 antibody (ab154253)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)

Key features and details

  • Rabbit polyclonal to PAX6
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PAX6 antibody
    See all PAX6 primary antibodies
  • Description

    Rabbit polyclonal to PAX6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-251 of Human PAX6 (UniProt ID: P26367).

  • Positive control

    • A431 and HepG2 whole cell lysate (ab7900); A549 cells and 293T cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/1000.
IP
1/500 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/1000.
IP
1/500 - 1/1000.

Target

  • Function

    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
  • Tissue specificity

    Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
  • Involvement in disease

    Defects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
    Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
    Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
    Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
    Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
    Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
    Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
    Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • Developmental stage

    Expressed in the developing eye and brain.
  • Post-translational
    modifications

    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P26367 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5080 Human
    • Omim: 607108 Human
    • SwissProt: P26367 Human
    • Unigene: 270303 Human
    • Unigene: 611376 Human
    • Alternative names

      • AN 2 antibody
      • AN antibody
      • AN2 antibody
      • Aniridia type II protein antibody
      • D11S812E antibody
      • FVH1 antibody
      • KIAA0552 antibody
      • Leucine zipper putative tumor suppressor 3 antibody
      • LZTS3 antibody
      • MGC17209 antibody
      • MGDA antibody
      • Oculorhombin antibody
      • Paired box 6 antibody
      • Paired box gene 6 (aniridia keratitis) antibody
      • Paired Box Gene 6 antibody
      • Paired box homeotic gene 6 antibody
      • Paired box protein Pax-6 antibody
      • Paired box protein Pax6 antibody
      • PAX 6 antibody
      • PAX6 antibody
      • PAX6_HUMAN antibody
      • ProSAP-interacting protein 1 antibody
      • PROSAPIP1 antibody
      • Sey antibody
      • WAGR antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
      Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
      Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling PAX6 with ab154253 at 1/200 dilution. The image in the lower panel was merged with DNA probe.
    • Western blot - Anti-PAX6 antibody (ab154253)
      Western blot - Anti-PAX6 antibody (ab154253)
      All lanes : Anti-PAX6 antibody (ab154253) at 1/1000 dilution

      Lane 1 : A431 whole cell lysate
      Lane 2 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 47 kDa

    • Immunoprecipitation - Anti-PAX6 antibody (ab154253)
      Immunoprecipitation - Anti-PAX6 antibody (ab154253)
      Immunoprecipitation of PAX6 using 2 µg of ab154253, and detected using ab154253 at 1/1000 dilution.
      Lane 1: 1000 µg 293T whole cell lysate.
      Lane 2: 50 µg 293T whole cell lysate
      Lane 3: 2 µg preimmune control Rabbit IgG

      10% SDS-PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
      Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab154253)
      Immunofluorescent analysis of paraformaldehyde-fixed 293T cells labeling PAX6 with ab154253 at 1/250 dilution.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab154253? Please let us know so that we can cite the reference in this datasheet.

    ab154253 has been referenced in 1 publication.

    • Guo Y  et al. Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities. Front Cell Neurosci 13:361 (2019). PubMed: 31481876

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