RecombinantRabMAb

Recombinant Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
  • Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15858] to PAX6 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAX6 antibody [EPR15858] - BSA and Azide free
    See all PAX6 primary antibodies

  • Description

    Rabbit monoclonal [EPR15858] to PAX6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment aa 150-350. The exact sequence is proprietary.
    Database link: P26367

  • General notes

    Ab233027 is the carrier-free version of ab195045. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab233027 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab233027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 47, 33, 32 kDa (predicted molecular weight: 47 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.

  • Tissue specificity

    Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

  • Involvement in disease

    Defects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
    Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
    Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
    Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
    Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
    Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
    Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
    Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.

  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.

  • Developmental stage

    Expressed in the developing eye and brain.

  • Post-translational
    modifications

    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AN 2 antibody
    • AN antibody
    • AN2 antibody
    • Aniridia type II protein antibody
    • D11S812E antibody
    • FVH1 antibody
    • KIAA0552 antibody
    • Leucine zipper putative tumor suppressor 3 antibody
    • LZTS3 antibody
    • MGC17209 antibody
    • MGDA antibody
    • Oculorhombin antibody
    • Paired box 6 antibody
    • Paired box gene 6 (aniridia keratitis) antibody
    • Paired Box Gene 6 antibody
    • Paired box homeotic gene 6 antibody
    • Paired box protein Pax-6 antibody
    • Paired box protein Pax6 antibody
    • PAX 6 antibody
    • PAX6 antibody
    • PAX6_HUMAN antibody
    • ProSAP-interacting protein 1 antibody
    • PROSAPIP1 antibody
    • Sey antibody
    • WAGR antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    IHC image of Pax6 staining in a formalin fixed, paraffin embedded normal rat cerebellum tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab195045, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    IHC image of Pax6 staining in a formalin fixed, paraffin embedded normal human cerebellum tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab195045, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 Neuro-2a (mouse neuroblastoma) cells labeling PAX6 with ab195045 at 1/350, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 (green).

    Confocal image showing cytoplasmic and nuclear staining on Neuro-2a cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 (red).  

    The negative controls are as follows:-

    -ve control 1: ab195045 at 1/350 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.

    -ve control 2: ab7291 at 1/500 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 Y79 (Human retinoblastoma cell line) cells labeling PAX6 with ab195045 at 1/350, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 (green).

    Confocal image showing cytoplasmic and nuclear staining on Y79 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 (red).  

    The negative controls are as follows:-
    -ve control 1: ab195045 at 1/350 followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500.

    -ve control 2: ab7291 at 1/500 followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PAX6 with ab195045 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Nuclear staining on human pancreas tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling PAX6 with ab195045 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Nuclear staining on human retina tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

    Immunohistochemical analysis of paraffin-embedded Human retinoblastoma tissue labeling PAX6 with ab195045 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Nuclear staining on human retinoblastoma tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195045).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)
    Anti-PAX6 antibody [EPR15858] - BSA and Azide free (ab233027)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab233027? Please let us know so that we can cite the reference in this datasheet.

ab233027 has not yet been referenced specifically in any publications.

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