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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-PAX6 antibody [PAX6496] (ab197768)

  • Datasheet
  • SDS
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Western blot - Anti-PAX6 antibody [PAX6496] (ab197768)

    Key features and details

    • Mouse monoclonal [PAX6496] to PAX6
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-PAX6 antibody [PAX6496]
      See all PAX6 primary antibodies
    • Description

      Mouse monoclonal [PAX6496] to PAX6
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken
    • Immunogen

      Recombinant fragment corresponding to Human PAX6.
      Database link: P26367

    • Positive control

      • WB: Human cerebellum tissue.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.05% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      PAX6496
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Neural Stem Cell marker
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • PAX
      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Stem Cells
      • Lineage Markers
      • Endoderm
      • Developmental Biology
      • Lineage specification
      • Endoderm
      • Developmental Biology
      • Lineage specification
      • Ectoderm
      • Developmental Biology
      • Organogenesis
      • Gut development
      • Pancreas development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Related Products

      • Recombinant Human PAX6 protein (ab134885)

    Applications

    Our Abpromise guarantee covers the use of ab197768 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 47 kDa.

    Target

    • Function

      Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
    • Tissue specificity

      Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
    • Involvement in disease

      Defects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
      Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
      Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
      Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
      Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
      Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
      Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
      Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
    • Sequence similarities

      Belongs to the paired homeobox family.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 paired domain.
    • Developmental stage

      Expressed in the developing eye and brain.
    • Post-translational
      modifications

      Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P26367 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5080 Human
      • Entrez Gene: 18508 Mouse
      • Entrez Gene: 25509 Rat
      • Omim: 607108 Human
      • SwissProt: P26367 Human
      • SwissProt: P63015 Mouse
      • SwissProt: P63016 Rat
      • Unigene: 270303 Human
      • Unigene: 611376 Human
      • Unigene: 33870 Mouse
      • Unigene: 3608 Mouse
      • Unigene: 89724 Rat
      see all
    • Alternative names

      • AN 2 antibody
      • AN antibody
      • AN2 antibody
      • Aniridia type II protein antibody
      • D11S812E antibody
      • FVH1 antibody
      • KIAA0552 antibody
      • Leucine zipper putative tumor suppressor 3 antibody
      • LZTS3 antibody
      • MGC17209 antibody
      • MGDA antibody
      • Oculorhombin antibody
      • Paired box 6 antibody
      • Paired box gene 6 (aniridia keratitis) antibody
      • Paired Box Gene 6 antibody
      • Paired box homeotic gene 6 antibody
      • Paired box protein Pax-6 antibody
      • Paired box protein Pax6 antibody
      • PAX 6 antibody
      • PAX6 antibody
      • PAX6_HUMAN antibody
      • ProSAP-interacting protein 1 antibody
      • PROSAPIP1 antibody
      • Sey antibody
      • WAGR antibody
      see all

    Images

    • Western blot - Anti-PAX6 antibody [PAX6496] (ab197768)
      Western blot - Anti-PAX6 antibody [PAX6496] (ab197768)
      Anti-PAX6 antibody [PAX6496] (ab197768) at 1 µg/ml + Human cerebellum tissue lysate

      Predicted band size: 47 kDa

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab197768? Please let us know so that we can cite the reference in this datasheet.

    ab197768 has been referenced in 1 publication.

    • Zou Q  et al. MicroRNA-375 targets PAX6 and inhibits the viability, migration and invasion of human breast cancer MCF-7 cells. Exp Ther Med 14:1198-1204 (2017). PubMed: 28810579

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