Anti-PAX7 antibody (ab34360)
Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAX7 antibody
See all PAX7 primary antibodies -
Description
Rabbit polyclonal to PAX7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
Read Abcam's proprietary immunogen policy (Peptide available as ab37576.) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Probable transcription factor. May have a role in myogenesis. -
Involvement in disease
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5081 Human
- Omim: 167410 Human
- SwissProt: P23759 Human
- Unigene: 113253 Human
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Alternative names
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
Images
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Western blot - Anti-PAX7 antibody (ab34360)All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml
Lane 1 : Human Uterus Tissue Lysate (negative control)
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Blocking peptides at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Protocols
References (31)
ab34360 has been referenced in 31 publications.
- Silva WJ et al. miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes. Acta Physiol (Oxf) 226:e13278 (2019). PubMed: 30943315
- Goldman SM et al. Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury. PLoS One 13:e0191245 (2018). PubMed: 29329332
- Silva JMS et al. Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection. PLoS One 13:e0196682 (2018). PubMed: 29709011
- Koide M et al. Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration. Tohoku J Exp Med 244:15-24 (2018). PubMed: 29311489
- Chaweewannakorn C et al. Roles of IL-1a/ß in regeneration of cardiotoxin-injured muscle and satellite cell function. Am J Physiol Regul Integr Comp Physiol 315:R90-R103 (2018). PubMed: 29513560
