Overview

  • Product name

  • Description

    Rabbit polyclonal to PAX7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.

    Read Abcam's proprietary immunogen policy (Peptide available as ab37576.)

  • Positive control

    • ab34360 was positive in the following Whole Cell Lysates: TE671 (Human Rhabdomyosarcoma cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab34360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 20723755
IHC-Fr Use at an assay dependent concentration. PubMed: 22253929

Target

  • Function

    Probable transcription factor. May have a role in myogenesis.
  • Involvement in disease

    Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Images

  • All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml

    Lane 1 : Human Uterus Tissue Lysate (negative control)
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Blocking peptides at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

This product has been referenced in:

  • Goldman SM  et al. Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury. PLoS One 13:e0191245 (2018). Read more (PubMed: 29329332) »
  • Silva JMS  et al. Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection. PLoS One 13:e0196682 (2018). Read more (PubMed: 29709011) »
See all 28 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skeletal muscle)
Permeabilization
No
Specification
skeletal muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 21 2017

Question
Answer

Thank you for contacting us.

Unfortunately, we do not carry a conjugated antibody for PAX7.

However, we carry a number of easy-to-use conjugation kits. Please see the link for the list of kits we carry:

https://www.abcam.com/EasyLink or https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

If you decide to use one of the kits, please check the purity of your antibody first to ensure efficient labeling.
Please also see: https://www.abcam.com/index.html?pageconfig=resource&rid=13489

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for your phone call.

I am going to contact the lab shortly regarding ab47052.

The link to our EasyLink conjugation kits can be found here: https://www.abcam.com/Easylink

For the information regarding the discount codes please see below:

DISCOUNT CODE: xxx for ab34360
DISCOUNT CODE: xxx for ab16148
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab34360 and ab16148 in flow cytometry. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for flow cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary mouse cells)
Loading amount
20 µg
Specification
primary mouse cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 19 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (muscle sections)
Specification
muscle sections
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.1% in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jun 30 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver (human mouse and rat) and mouse small bowel)
Specification
liver (human mouse and rat) and mouse small bowel
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Jun 04 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12)
Loading amount
20 µg
Specification
HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 07 2008

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