Anti-PAX7 antibody (ab34360)
Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PAX7 antibody
See all PAX7 primary antibodies -
Description
Rabbit polyclonal to PAX7 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
Read Abcam's proprietary immunogen policy (Peptide available as ab37576.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). |
Target
-
Function
Probable transcription factor. May have a role in myogenesis. -
Involvement in disease
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5081 Human
- Omim: 167410 Human
- SwissProt: P23759 Human
- Unigene: 113253 Human
-
Alternative names
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
Images
-
Western blot - Anti-PAX7 antibody (ab34360)All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml
Lane 1 : Human Uterus Tissue Lysate (negative control)
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Blocking peptides at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (35)
ab34360 has been referenced in 35 publications.
- Malatesta M et al. Satellite Cells in Skeletal Muscle of the Hibernating Dormouse, a Natural Model of Quiescence and Re-Activation: Focus on the Cell Nucleus. Cells 9:N/A (2020). PubMed: 32340154
- Guo X et al. Characterization of Functional Human Skeletal Myotubes and Neuromuscular Junction Derived-From the Same Induced Pluripotent Stem Cell Source. Bioengineering (Basel) 7:N/A (2020). PubMed: 33105732
- Al Tanoury Z et al. Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development 147:N/A (2020). PubMed: 32541004
- Silva WJ et al. miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes. Acta Physiol (Oxf) 226:e13278 (2019). PubMed: 30943315
- Jama A et al. Lipin1 is required for skeletal muscle development by regulating MEF2c and MyoD expression. J Physiol N/A:N/A (2018). PubMed: 30511745