Key features and details
- Rabbit polyclonal to PAX7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAX7 antibody
See all PAX7 primary antibodies
DescriptionRabbit polyclonal to PAX7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).|
FunctionProbable transcription factor. May have a role in myogenesis.
Involvement in diseaseDefects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.
- Information by UniProt
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml
Lane 1 : Human Uterus Tissue Lysate (negative control)
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Blocking peptides at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab34360 has been referenced in 31 publications.
- Silva WJ et al. miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes. Acta Physiol (Oxf) 226:e13278 (2019). PubMed: 30943315
- Goldman SM et al. Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury. PLoS One 13:e0191245 (2018). PubMed: 29329332
- Silva JMS et al. Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection. PLoS One 13:e0196682 (2018). PubMed: 29709011
- Koide M et al. Retained Myogenic Potency of Human Satellite Cells from Torn Rotator Cuff Muscles Despite Fatty Infiltration. Tohoku J Exp Med 244:15-24 (2018). PubMed: 29311489
- Chaweewannakorn C et al. Roles of IL-1a/ß in regeneration of cardiotoxin-injured muscle and satellite cell function. Am J Physiol Regul Integr Comp Physiol 315:R90-R103 (2018). PubMed: 29513560