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    pax7-antibody-ab34360.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families PAX
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Anti-PAX7 antibody (ab34360)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (35)

Product price, shipping and contact information

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Abpromise

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Find out more.

Western blot - Anti-PAX7 antibody (ab34360)

    Key features and details

    • Rabbit polyclonal to PAX7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-PAX7 antibody [EPR3353] (ab92317)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-PAX7 antibody
      See all PAX7 primary antibodies
    • Description

      Rabbit polyclonal to PAX7
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.

      Read Abcam's proprietary immunogen policy (Peptide available as ab37576.)
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • PAX
      • Stem Cells
      • Lineage Markers
      • Ectoderm
      • Stem Cells
      • Mesenchymal Stem Cells
      • Myogenesis
      • Developmental Biology
      • Lineage specification
      • Ectoderm
      • Developmental Biology
      • Organogenesis
      • Skeletal development
      • Muscle

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant Human PAX7 protein (ab114237)
    • Recombinant Protein

      • Recombinant Human PAX7 protein (ab114237)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34360 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (2)
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa).

    Target

    • Function

      Probable transcription factor. May have a role in myogenesis.
    • Involvement in disease

      Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
    • Sequence similarities

      Belongs to the paired homeobox family.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 paired domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P23759 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5081 Human
      • Omim: 167410 Human
      • SwissProt: P23759 Human
      • Unigene: 113253 Human
      • Alternative names

        • FLJ37460 antibody
        • HUP1 antibody
        • OTTHUMP00000002534 antibody
        • Paired box 7 antibody
        • Paired box gene 7 antibody
        • Paired box homeotic gene 7 antibody
        • Paired box protein Pax-7 antibody
        • Paired domain gene 7 antibody
        • Paired domain gene HuP1 antibody
        • Pax7 antibody
        • PAX7 transcriptional factor antibody
        • PAX7/FKHR fusion gene, included antibody
        • PAX7_HUMAN antibody
        • PAX7B antibody
        • RGD1564360 antibody
        • RMS2 antibody
        see all

      Images

      • Western blot - Anti-PAX7 antibody (ab34360)
        Western blot - Anti-PAX7 antibody (ab34360)
        All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml

        Lane 1 : Human Uterus Tissue Lysate (negative control)
        Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Blocking peptides at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 57 kDa
        Observed band size: 65 kDa why is the actual band size different from the predicted?
        Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 2 minutes


        This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (35)

      Publishing research using ab34360? Please let us know so that we can cite the reference in this datasheet.

      ab34360 has been referenced in 35 publications.

      • Malatesta M  et al. Satellite Cells in Skeletal Muscle of the Hibernating Dormouse, a Natural Model of Quiescence and Re-Activation: Focus on the Cell Nucleus. Cells 9:N/A (2020). PubMed: 32340154
      • Guo X  et al. Characterization of Functional Human Skeletal Myotubes and Neuromuscular Junction Derived-From the Same Induced Pluripotent Stem Cell Source. Bioengineering (Basel) 7:N/A (2020). PubMed: 33105732
      • Al Tanoury Z  et al. Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage in vitro. Development 147:N/A (2020). PubMed: 32541004
      • Silva WJ  et al. miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes. Acta Physiol (Oxf) 226:e13278 (2019). PubMed: 30943315
      • Jama A  et al. Lipin1 is required for skeletal muscle development by regulating MEF2c and MyoD expression. J Physiol N/A:N/A (2018). PubMed: 30511745
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-PAX7 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (skeletal muscle)
      Permeabilization
      No
      Specification
      skeletal muscle
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 21 2017

      Western blot abreview for Anti-PAX7 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (primary mouse cells)
      Loading amount
      20 µg
      Specification
      primary mouse cells
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 19 2010

      Immunohistochemistry (Frozen sections) abreview for Anti-PAX7 antibody

      Inconclusive
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (muscle sections)
      Specification
      muscle sections
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - Triton X-100 0.1% in PBS
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 30 2010

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PAX7 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (liver (human mouse and rat) and mouse small bowel)
      Specification
      liver (human mouse and rat) and mouse small bowel
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      No
      Blocking step
      Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
      Read More

      Mr. Antibody Solutions

      Verified customer

      Submitted Jun 04 2008

      Western blot abreview for Anti-PAX7 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12)
      Loading amount
      20 µg
      Specification
      HeLa (Hu), HEK293 (Hu), NIH-3T3, PC-12
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Mr. Antibody Solutions

      Verified customer

      Submitted Apr 07 2008

      Question

      send information about conjugation kits

      Read More

      Abcam community

      Verified customer

      Asked on May 29 2012

      Answer

      Thank you for contacting us.

      Unfortunately, we do not carry a conjugated antibody for PAX7.

      However, we carry a number of easy-to-use conjugation kits. Please see the link for the list of kits we carry:

      https://www.abcam.com/EasyLink or https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

      If you decide to use one of the kits, please check the purity of your antibody first to ensure efficient labeling.
      Please also see: https://www.abcam.com/index.html?pageconfig=resource&rid=13489

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on May 29 2012

      Question

      testing ab34360 and ab16148 in flow cytometry
      ab47052: check emission wavelength
      send link to EasyLink kit page

      Read More

      Abcam community

      Verified customer

      Asked on Apr 13 2012

      Answer

      Thank you for your phone call.

      I am going to contact the lab shortly regarding ab47052.

      The link to our EasyLink conjugation kits can be found here: https://www.abcam.com/Easylink

      For the information regarding the discount codes please see below:

      DISCOUNT CODE: xxx for ab34360
      DISCOUNT CODE: xxx for ab16148
      Expiration date: xxx

      I am very pleased to hear you would like to accept our offer and test ab34360 and ab16148 in flow cytometry. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for flow cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Apr 13 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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