- Datasheet
- Specific References (25)
- Protocols
Overview
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Product nameAnti-PAX7 antibody
See all PAX7 primary antibodies -
DescriptionRabbit polyclonal to PAX7
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Frmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
(Peptide available as ab37576.)
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Positive control
- ab34360 was positive in the following Whole Cell Lysates: TE671 (Human Rhabdomyosarcoma cell line).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 57 kDa). | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 20723755 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22253929 |
Target
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FunctionProbable transcription factor. May have a role in myogenesis.
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Involvement in diseaseDefects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
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Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5081 Human
- Omim: 167410 Human
- SwissProt: P23759 Human
- Unigene: 113253 Human
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Alternative names
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
Images
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Western blot - Anti-PAX7 antibody (ab34360)All lanes : Anti-PAX7 antibody (ab34360) at 1 µg/ml
Lane 1 : Human Uterus Tissue Lysate (negative control)
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Blocking peptides at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 65 kDa (why is the actual band size different from the predicted?)
Additional bands at: 37 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Protocols
References
This product has been referenced in:
- Goldman SM et al. Co-delivery of a laminin-111 supplemented hyaluronic acid based hydrogel with minced muscle graft in the treatment of volumetric muscle loss injury. PLoS One 13:e0191245 (2018). Read more (PubMed: 29329332) »
- Silva JMS et al. Muscle wasting in osteoarthritis model induced by anterior cruciate ligament transection. PLoS One 13:e0196682 (2018). Read more (PubMed: 29709011) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"