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Inquiry: We can see that others have requested the flowcytometric protocol as well as the isotype control. We would like that as well if possible? Also, have you performed any analysis on muscle cells? since these are the cells to express PAX7. You use three cell lines that I would not expect to express PAX7?
Asked on Jan 22 2013
Thank you for your inquiry.
We permeabilized the HeLa cells with 1X FACS permeabilizing solution for 10 minutes and blocked with 0.5% BSA in 1X PBS for 30 minutes. We used a 1:200 primary dilution (30 min at room temperature). We use a FITC AffiniPure goat anti-rabbit IgG (H+L) secondary.
If you need any further assistance in the future, please do not hesitate to contact me.
Answered on Jan 22 2013