Anti-PAX7 antibody [mAbcam61067] (ab61067)

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Western blot - Anti-PAX7 antibody [mAbcam61067] (ab61067)
  • Flow Cytometry - Anti-PAX7 antibody [mAbcam61067] (ab61067)

Key features and details

  • Mouse monoclonal [mAbcam61067] to PAX7
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-PAX7 antibody [mAbcam61067]
    See all PAX7 primary antibodies

  • Description

    Mouse monoclonal [mAbcam61067] to PAX7

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse PAX7 aa 200-300.
    (Peptide available as ab112031)

  • Positive control

    • This antibody gave a positive signal in HeLa and NIH3T3 whole cell lysates.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab61067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Mouse
IHC-Fr
Mouse
Rat
IHC-P
Mouse
Rat
WB
Mouse
Human
All applications
Rat
Chicken
Cow
Turkey
Chimpanzee
Zebrafish
Gorilla
Orangutan
Application Abreviews Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
Notes
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Flow Cyt
Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Probable transcription factor. May have a role in myogenesis.

  • Involvement in disease

    Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.

  • Sequence similarities

    Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ37460 antibody
    • HUP1 antibody
    • OTTHUMP00000002534 antibody
    • Paired box 7 antibody
    • Paired box gene 7 antibody
    • Paired box homeotic gene 7 antibody
    • Paired box protein Pax-7 antibody
    • Paired domain gene 7 antibody
    • Paired domain gene HuP1 antibody
    • Pax7 antibody
    • PAX7 transcriptional factor antibody
    • PAX7/FKHR fusion gene, included antibody
    • PAX7_HUMAN antibody
    • PAX7B antibody
    • RGD1564360 antibody
    • RMS2 antibody
    see all

Images

  • Western blot - Anti-PAX7 antibody [mAbcam61067] (ab61067)
    Western blot - Anti-PAX7 antibody [mAbcam61067] (ab61067)
    All lanes : Anti-PAX7 antibody [mAbcam61067] (ab61067) at 2 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 37 kDa, 40 kDa, 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Flow Cytometry - Anti-PAX7 antibody [mAbcam61067] (ab61067)
    Flow Cytometry - Anti-PAX7 antibody [mAbcam61067] (ab61067)
    Overlay histogram showing HepG2 cells stained with ab61067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61067, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References (0)

Publishing research using ab61067? Please let us know so that we can cite the reference in this datasheet.

ab61067 has not yet been referenced specifically in any publications.

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