Product nameAnti-PAX7 antibody [PAX7497]
See all PAX7 primary antibodies
DescriptionMouse monoclonal [PAX7497] to PAX7
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Zebrafish
Recombinant full length protein corresponding to Human PAX7 aa 1-520.
MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRP LPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGA IGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPS GLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLD EGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREEL AQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTL PPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSS AYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQP QADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTT GYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFMESYKVVSG WGMSISQMEKLKSSQMEQFT
Database link: P23759
- HeLa, Jurkat, A431, MCF-7, U2OS and NIH/3T3 cells. Stomach or rhabdomyosarcoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab199010 in the following tested applications.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at 1-2 µg/mg of lysate.|
FunctionProbable transcription factor. May have a role in myogenesis.
Involvement in diseaseDefects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.
- Information by UniProt
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
Anti-PAX7 antibody [PAX7497] (ab199010) at 1 µg/ml + HeLa cell lysate
Predicted band size: 57 kDa
Ab199010 staining PAX7 in Mouse tibialis tissue by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 100% CAS block for 1 hour at 26°C; permeabilized with tween. Samples were incubated the primary antibody (1/150 in PBS + Tween) for 16 hours at 4°C. An Alexa Fluor® 488 Goat polyclonal (1/1000 dilution) was used as the secondary antibody.
This product has been referenced in:
- Syverud BC et al. Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation. Tissue Eng Part C Methods 24:32-41 (2018). Read more (PubMed: 28946802) »
- Wang QQ et al. Human Umbilical Cord Wharton's Jelly Derived Mesenchymal Stromal Cells May Attenuate Sarcopenia in Aged Mice Induced by Hindlimb Suspension. Med Sci Monit 24:9272-9281 (2018). Read more (PubMed: 30571669) »