Key features and details
- Mouse monoclonal [PAX7497] to PAX7
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Chicken, Human, Zebrafish
- Isotype: IgG1
Product nameAnti-PAX7 antibody [PAX7497]
See all PAX7 primary antibodies
DescriptionMouse monoclonal [PAX7497] to PAX7
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Zebrafish
Recombinant full length protein corresponding to Human PAX7 aa 1-520.
MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRP LPNHIRHKIVEMAHHGIRPCVISRQLRVSHGCVSKILCRYQETGSIRPGA IGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKDGHCDRSTVPS GLVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLD EGSDVESEPDLPLKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREEL AQRTKLTEARVQVWFSNRRARWRKQAGANQLAAFNHLLPGGFPPTGMPTL PPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSS AYGARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQP QADFSISPLHGGLDSATSISASCSQRADSIKPGDSLPTSQAYCPPTYSTT GYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFMESYKVVSG WGMSISQMEKLKSSQMEQFT
Database link: P23759
- HeLa, Jurkat, A431, MCF-7, U2OS and NIH/3T3 cells. Stomach or rhabdomyosarcoma.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab199010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionProbable transcription factor. May have a role in myogenesis.
Involvement in diseaseDefects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.
- Information by UniProt
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
ab199010 has been referenced in 2 publications.
- Syverud BC et al. Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation. Tissue Eng Part C Methods 24:32-41 (2018). PubMed: 28946802
- Wang QQ et al. Human Umbilical Cord Wharton's Jelly Derived Mesenchymal Stromal Cells May Attenuate Sarcopenia in Aged Mice Induced by Hindlimb Suspension. Med Sci Monit 24:9272-9281 (2018). PubMed: 30571669