Anti-PAX7 antibody [PAX7497] (ab199010)
Key features and details
- Mouse monoclonal [PAX7497] to PAX7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PAX7 antibody [PAX7497]
See all PAX7 primary antibodies -
Description
Mouse monoclonal [PAX7497] to PAX7 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human PAX7 aa 1 to the C-terminus.
Database link: P23759 -
Positive control
- HeLa, Jurkat, A431, MCF-7, U2OS and NIH/3T3 cells. Stomach or rhabdomyosarcoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
PAX7497 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa. |
Target
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Function
Probable transcription factor. May have a role in myogenesis. -
Involvement in disease
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5081 Human
- Omim: 167410 Human
- SwissProt: P23759 Human
- Unigene: 113253 Human
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Alternative names
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab199010 has been referenced in 13 publications.
- Cefis M et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
- Vicario N et al. Clobetasol promotes neuromuscular plasticity in mice after motoneuronal loss via sonic hedgehog signaling, immunomodulation and metabolic rebalancing. Cell Death Dis 12:625 (2021). PubMed: 34135312
- Grunow B et al. Histological and biochemical evaluation of skeletal muscle in the two salmonid species Coregonus maraena and Oncorhynchus mykiss. PLoS One 16:e0255062 (2021). PubMed: 34383783
- Shang GK et al. Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRa+ mesenchymal progenitors. J Cachexia Sarcopenia Muscle N/A:N/A (2021). PubMed: 34435457
- Ling X et al. Lidocaine Inhibits Myoblast Cell Migration and Myogenic Differentiation Through Activation of the Notch Pathway. Drug Des Devel Ther 15:927-936 (2021). PubMed: 33688167