Key features and details
- Goat polyclonal to PAX8
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PAX8 antibody
See all PAX8 primary antibodies
DescriptionGoat polyclonal to PAX8
SpecificityThis antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents.
Tested applicationsSuitable for: IHC-Fr, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- IHC-P: Human thyroid tissue WB: Human kidney tissue lysate, HeLa cell lysate and Human thyroid cells
General notesGenBank Accession Number - NP_003457; NP_039245; NP_039246; NP_039247; NP_054698
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab13611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|WB||Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
Sequence similaritiesContains 1 paired domain.
Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
- Information by UniProt
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.
All lanes : Anti-PAX8 antibody (ab13611) at 1 µg/ml
Lane 1 : Human Kidney tissue lysate (35µg protein in RIPA buffer).
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer).
Predicted band size: 48 kDa
Detected by chemiluminescence.
ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection.
Nuclear extract of PC Cl3 thyroid cells (15
g) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). µ
PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.
The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).
This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.
Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at
ab13611 has been referenced in 7 publications.
- O'Brien LL et al. Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies. PLoS Genet 14:e1007181 (2018). PubMed: 29377931
- Zane M et al. Estrogen and thyroid cancer is a stem affair: A preliminary study. Biomed Pharmacother 85:399-411 (2017). Flow Cyt, ICC/IF . PubMed: 27899250
- Arauchi A et al. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture. Front Endocrinol (Lausanne) 8:103 (2017). PubMed: 28588551
- Kang HM et al. Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice. Cell Rep 14:861-871 (2016). PubMed: 26776520
- Sicking EM et al. Subtotal ablation of parietal epithelial cells induces crescent formation. J Am Soc Nephrol 23:629-40 (2012). IHC-P ; Mouse . PubMed: 22282596
- Borgogni E et al. Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells. Endocrinology 149:3626-34 (2008). PubMed: 18372324
- Crescioli C et al. Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. J Endocrinol 195:145-55 (2007). PubMed: 17911406