Overview

  • Product name
  • Description
    Goat polyclonal to PAX8
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognise all five human isoforms (as represented by NP_003457; NP_039245; NP_039246; NP_039247; NP_054698). This antibody detects a strong band at approx 40kDa in Human Liver lysates. The identity of this band is unclear given the existence of 5 isoforms of PAX8 between 450 aa and 287 aa (54-34 kD). It has shown to be specific for PAX8 in experiments with nuclear extracts of PC Cl3 thyroid cells cultured for 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) for 24 h (TSH stimulated cells). A band representing PAX8 is seen in the stimulated cells at 60kD (although other non-specific background bands are also seen). We have no confirmation of which isoform this represents.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide: PHNSIRSGHGGLN, corresponding to N terminal amino acids 2-14 of Human PAX8.

  • Positive control
    • IHC-P: Human thyroid and kidney tissue WB: Human liver and thyroid cells
  • General notes
    GenBank Accession Number - NP_003457; NP_039245; NP_039246; NP_039247; NP_054698

Properties

Applications

Our Abpromise guarantee covers the use of ab13611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 2 - 4 µg/ml.
WB Use a concentration of 1.5 - 4.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human PAX8 peptide (ab23220).

Target

  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney cortex tissue staining PAX8 with ab13611 at 5µg/ml. Heat-mediated antigen retrieval with citrate buffer (ph 6.0) was performed. Antibody was revealed with AP-staining.

  • ab13611 (2µg/ml) staining of paraffin embedded Human Human Thyroid Gland following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining shows nuclear stains in the epithelial cells around the follicles.

  • Anti-PAX8 antibody (ab13611) at 1 µg/ml + Human liver lysate (in RIPA buffer) at 35 µg

    Predicted band size: 48 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • ab13611 at 3µg/ml staining PAX8 in human thyroid gland sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by microwave in Tris/EDTA buffer pH 9.0. The HRP-staining procedure was used for detection. 

  • Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells).

    PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands.

    The indicated PAX8 band is at 60 kD. (In some gels PAX8 runs at 55-58kD).

    This image was kindly supplied as part of the review submitted by Dr Pilar Santisteban.

    Nuclear extract of PC Cl3 thyroid cells (15 µg) cultured during 7 days without stimulation from serum and thyrotropin (starved cells) and then stimulated with thyrotropin (TSH) 0.5 mUI/ml during 24 h (TSH stimulated cells). PAX8 is seen to be correctly regulated in panel A. Panel B shows an extended view of the same gel with other visible background bands. The indicated PAX8 band is at

References

This product has been referenced in:
  • O'Brien LL  et al. Transcriptional regulatory control of mammalian nephron progenitors revealed by multi-factor cistromic analysis and genetic studies. PLoS Genet 14:e1007181 (2018). Read more (PubMed: 29377931) »
  • Zane M  et al. Estrogen and thyroid cancer is a stem affair: A preliminary study. Biomed Pharmacother 85:399-411 (2017). Flow Cyt, ICC/IF . Read more (PubMed: 27899250) »
See all 7 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% acrylamide)
Sample
Human Cell lysate - whole cell (renal cancer cell line)
Specification
renal cancer cell line
Blocking step
Licor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 30 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Cell (Kidney tissue)
Specification
Kidney tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton x100
Blocking step
Serum as blocking agent for 4 minute(s) · Concentration: 5.26% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 13 2012

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