Overview

  • Product name
    Anti-PAX8 antibody
    See all PAX8 primary antibodies
  • Description
    Rabbit polyclonal to PAX8
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Xenopus laevis, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).

  • Positive control
    • WB: 293T, H1299, HepG2, MOLT4 or Raji cell lysate; ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab97477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • Western blot - Anti-PAX8 antibody (ab97477)
    Western blot - Anti-PAX8 antibody (ab97477)
    Anti-PAX8 antibody (ab97477) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 48 kDa



    10% SDS page
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)
    Top: immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab97477 antibody at 1/200 dilution.
    Bottom: PAX8 stain merged with DNA probe stain.

References

This product has been referenced in:
  • Perny M  et al. Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture. Front Cell Neurosci 11:409 (2017). ICC/IF . Read more (PubMed: 29311837) »
  • DeJonge RE  et al. Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS One 11:e0162508 (2016). IHC-P . Read more (PubMed: 27607106) »

See all 3 Publications for this product

Publishing research using ab97477? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-PAX8 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cell)
Permeabilization
Yes - 0,25% Triton X-100
Specification
differentiated induced pluripotent stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Read More
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Username

Abcam user community

Verified customer

Submitted Jun 23 2017

Western blot abreview for Anti-PAX8 antibody

 Below Average
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus tropicalis Cell lysate - whole cell (Whole embryo)
Loading amount
20 µg
Specification
Whole embryo
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
abreview image
Username

Dr. Florencia del Viso

Verified customer

Submitted May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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