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Anti-PAX8 antibody (ab97477)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (4)

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ChIP - Anti-PAX8 antibody (ab97477)
  • Western blot - Anti-PAX8 antibody (ab97477)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)
  • Immunoprecipitation - Anti-PAX8 antibody (ab97477)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)
  • Western blot - Anti-PAX8 antibody (ab97477)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

Key features and details

  • Rabbit polyclonal to PAX8
  • Suitable for: WB, ICC/IF, ChIP, IHC-P, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PAX8 antibody
    See all PAX8 primary antibodies
  • Description

    Rabbit polyclonal to PAX8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, ChIP, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).

  • Positive control

    • ChIP: HEK-293T cells; WB: HEK-293T whole cell lysate; IHC-P: DLD1 xenograft, mouse liver and rat thyroid gland tissue; IF: A549 whole cells. IP: HEK-293T whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Auditory system
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
    • Raji whole cell lysate (ab30124)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/2000. Predicted molecular weight: 48 kDa.
ICC/IF (1)
1/100 - 1/1000.
ChIP
Use at an assay dependent concentration.
IHC-P
1/100 - 1/1000.
IP
1/500 - 1/1000.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 48 kDa.
ICC/IF
1/100 - 1/1000.
ChIP
Use at an assay dependent concentration.
IHC-P
1/100 - 1/1000.
IP
1/500 - 1/1000.

Target

  • Function

    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity

    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease

    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q06710 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7849 Human
    • Entrez Gene: 18510 Mouse
    • Entrez Gene: 81819 Rat
    • Omim: 167415 Human
    • SwissProt: Q06710 Human
    • SwissProt: Q00288 Mouse
    • SwissProt: P51974 Rat
    • Unigene: 469728 Human
    • Unigene: 2533 Mouse
    • Unigene: 10392 Rat
    see all
  • Alternative names

    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • ChIP - Anti-PAX8 antibody (ab97477)
    ChIP - Anti-PAX8 antibody (ab97477)

    PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.

    ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;

    A. 5 μg preimmune rabbit IgG;

    B. 5 μg of ab97477.

    The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

  • Western blot - Anti-PAX8 antibody (ab97477)
    Western blot - Anti-PAX8 antibody (ab97477)
    All lanes : Anti-PAX8 antibody (ab97477) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : DDDDK-tagged PAX8 transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 48 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)
    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom: Costained with DNA probe stain.

  • Immunoprecipitation - Anti-PAX8 antibody (ab97477)
    Immunoprecipitation - Anti-PAX8 antibody (ab97477)

    ab97477 immunoprecipitates PAX8 protein in IP experiments.

    IP Sample: 1000 μg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    1- 40 μg HEK-293T whole cell lysate;

    2- Control with 2 μg of preimmune rabbit IgG;

    3- Immunoprecipitation of PAX8 protein by 2 μg ofab97477.

    10% SDS-PAGE

    The immunoprecipitated PAX8 protein was detected byab97477 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-PAX8 antibody (ab97477)
    Western blot - Anti-PAX8 antibody (ab97477)
    All lanes : Anti-PAX8 antibody (ab97477) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HEK-293T cell nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.

    Antigen Retrieval:EDTA based buffer, pH 8.0, 15 min.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab97477? Please let us know so that we can cite the reference in this datasheet.

ab97477 has been referenced in 4 publications.

  • Perny M  et al. Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture. Front Cell Neurosci 11:409 (2017). ICC/IF . PubMed: 29311837
  • DeJonge RE  et al. Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS One 11:e0162508 (2016). IHC-P . PubMed: 27607106
  • Ma R  et al. Human embryonic stem cells form functional thyroid follicles. Thyroid 25:455-61 (2015). PubMed: 25585054
  • Koehler KR & Hashino E 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat Protoc 9:1229-44 (2014). PubMed: 24784820

Customer reviews and Q&As

Show All Reviews Q&A
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Western blot abreview for Anti-PAX8 antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus tropicalis Cell lysate - whole cell (Whole embryo)
Loading amount
20 µg
Specification
Whole embryo
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Dr. Florencia del Viso

Verified customer

Submitted May 20 2011

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