Anti-PAX8 antibody (ab97477)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-PAX8 antibody
See all PAX8 primary antibodies -
DescriptionRabbit polyclonal to PAX8
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Xenopus laevis, Monkey
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).
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Positive control
- WB: 293T, H1299, HepG2, MOLT4 or Raji cell lysate; ICC/IF: A549 cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 48 kDa. | |
| ICC/IF | 1/100 - 1/200. |
Target
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FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
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Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
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Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
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Sequence similaritiesContains 1 paired domain.
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Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 403927 Dog
- Entrez Gene: 7849 Human
- Entrez Gene: 18510 Mouse
- Entrez Gene: 81819 Rat
- Entrez Gene: 398132 Xenopus laevis
- Omim: 167415 Human
- SwissProt: Q06710 Human
- SwissProt: Q00288 Mouse
see all -
Alternative names
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
Images
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Western blot - Anti-PAX8 antibody (ab97477)Anti-PAX8 antibody (ab97477) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS page -
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)Top: immunofluorescence analysis of paraformaldehyde-fixed A549 cells, using ab97477 antibody at 1/200 dilution.
Bottom: PAX8 stain merged with DNA probe stain.
References
This product has been referenced in:
- Perny M et al. Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture. Front Cell Neurosci 11:409 (2017). ICC/IF . Read more (PubMed: 29311837) »
- DeJonge RE et al. Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS One 11:e0162508 (2016). IHC-P . Read more (PubMed: 27607106) »
