Anti-PAX8 antibody (ab97477)
Key features and details
- Rabbit polyclonal to PAX8
- Suitable for: WB, ICC/IF, ChIP, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PAX8 antibody
See all PAX8 primary antibodies -
Description
Rabbit polyclonal to PAX8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIP, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 214 of PAX8 (Uniprot: Q06710).
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Positive control
- ChIP: HEK-293T cells; WB: HEK-293T whole cell lysate; IHC-P: DLD1 xenograft, mouse liver and rat thyroid gland tissue; IF: A549 whole cells. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 48 kDa.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| ChIP |
Use at an assay dependent concentration.
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| IHC-P |
1/100 - 1/1000.
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| IP |
1/500 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 48 kDa. |
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ICC/IF
1/100 - 1/1000. |
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ChIP
Use at an assay dependent concentration. |
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IHC-P
1/100 - 1/1000. |
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IP
1/500 - 1/1000. |
Target
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Function
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. -
Tissue specificity
Expressed in the excretory system, thyroid gland and Wilms tumors. -
Involvement in disease
Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. -
Sequence similarities
Contains 1 paired domain. -
Developmental stage
In developing excretory system, during thyroid differentiation and in adult thyroid. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7849 Human
- Entrez Gene: 18510 Mouse
- Entrez Gene: 81819 Rat
- Omim: 167415 Human
- SwissProt: Q06710 Human
- SwissProt: Q00288 Mouse
- SwissProt: P51974 Rat
- Unigene: 469728 Human
see all -
Alternative names
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
see all
Images
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ChIP - Anti-PAX8 antibody (ab97477)
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.
ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;
A. 5 μg preimmune rabbit IgG;
B. 5 μg of ab97477.
The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.
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Western blot - Anti-PAX8 antibody (ab97477)All lanes : Anti-PAX8 antibody (ab97477) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DDDDK-tagged PAX8 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 48 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom: Costained with DNA probe stain.
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Immunoprecipitation - Anti-PAX8 antibody (ab97477)ab97477 immunoprecipitates PAX8 protein in IP experiments.
IP Sample: 1000 μg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
1- 40 μg HEK-293T whole cell lysate;
2- Control with 2 μg of preimmune rabbit IgG;
3- Immunoprecipitation of PAX8 protein by 2 μg ofab97477.
10% SDS-PAGE
The immunoprecipitated PAX8 protein was detected byab97477 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Western blot - Anti-PAX8 antibody (ab97477)All lanes : Anti-PAX8 antibody (ab97477) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T cell nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 48 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.
Antigen Retrieval:EDTA based buffer, pH 8.0, 15 min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97477 has been referenced in 4 publications.
- Perny M et al. Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture. Front Cell Neurosci 11:409 (2017). ICC/IF . PubMed: 29311837
- DeJonge RE et al. Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture. PLoS One 11:e0162508 (2016). IHC-P . PubMed: 27607106
- Ma R et al. Human embryonic stem cells form functional thyroid follicles. Thyroid 25:455-61 (2015). PubMed: 25585054
- Koehler KR & Hashino E 3D mouse embryonic stem cell culture for generating inner ear organoids. Nat Protoc 9:1229-44 (2014). PubMed: 24784820