Anti-PAX8 antibody [BC12] (ab124445)

Mouse monoclonal PAX8 antibody [BC12]. Validated in WB, IHC and tested in Mouse, Rat, Cat, Dog, Human. Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-PAX8 antibody [BC12]
    See all PAX8 primary antibodies
  • Description

    Mouse monoclonal [BC12] to PAX8
  • Host species

    Mouse
  • Specificity

    ab124445 does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to PAX8.

  • Positive control

    • Human normal kidney, renal cell or serous ovarian carcinoma tissues.
  • General notes

    Make dilutions in Renoir Red diluent.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab124445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function

    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity

    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease

    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • ab124445 at 1/100 dilution staining PAX8 in formalin-fixed paraffin-embedded Human Renal cell carcinoma tissue by Immunohistochemistry.

References

ab124445 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (786-0, a renal cell carcinoma cell line)
Loading amount
50 µg
Specification
786-0, a renal cell carcinoma cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 26 2013

Answer

1) How was antibody specificity tested?

-We have done ELISA, Western Blot, and IHC for the specificity tests.


2) Do you have any information regarding cross-reactivity of this Pax8 antibody with other Pax-Proteins, especially Pax2 and Pax5?

-BC12 does not cross-react with other PAX family members, like Pax2 and Pax5.



3) Do you provide detailed information on the immunogen

-recombinant human PAX8



4) What is the epitope recognized by the antibody?

-Epitope: aa258-272 of human PAX8.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

Thank you for your patience.

The lab sent me the following information this afternoon:

The ab124445was raised by immunizing full length of human PAX8. The epitope maps to an internal sequence. There is no cross-reactivity with PAX2 and PAX5.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for contacting us.

The immunogen isthe recombinantfull-length human PAX8 protein. The exact epitope has not been mapped, therefore we do not know where the antibody would bind in this region.

As for the specificity and cross-reactivity with Pax2 and Pax5, I will check with the lab for potential data or additional information.

I hope this information is so far helpful to you. I will be in touch. Thank you for your patience.
In the meantime, please do not hesitate to contact us if you need any more advice or information.

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