Anti-PAX8 antibody [BC12] (ab124445)

Mouse monoclonal PAX8 antibody [BC12]. Validated in WB, IHC and tested in Mouse, Rat, Cat, Dog, Human. Independently reviewed in 1 review(s).


  • Product name

    Anti-PAX8 antibody [BC12]
    See all PAX8 primary antibodies
  • Description

    Mouse monoclonal [BC12] to PAX8
  • Host species

  • Specificity

    ab124445 does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cat, Dog, Human
  • Immunogen

    Other Immunogen Type corresponding to PAX8.

  • Positive control

    • Human normal kidney, renal cell or serous ovarian carcinoma tissues.
  • General notes

    Make dilutions in Renoir Red diluent.




Our Abpromise guarantee covers the use of ab124445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.


  • Function

    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity

    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease

    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities

    Contains 1 paired domain.
  • Developmental stage

    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all


  • ab124445 at 1/100 dilution staining PAX8 in formalin-fixed paraffin-embedded Human Renal cell carcinoma tissue by Immunohistochemistry.


ab124445 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (786-0, a renal cell carcinoma cell line)
Loading amount
50 µg
786-0, a renal cell carcinoma cell line
Gel Running Conditions
Reduced Denaturing (4-12% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 26 2013


1) How was antibody specificity tested?

-We have done ELISA, Western Blot, and IHC for the specificity tests.

2) Do you have any information regarding cross-reactivity of this Pax8 antibody with other Pax-Proteins, especially Pax2 and Pax5?

-BC12 does not cross-react with other PAX family members, like Pax2 and Pax5.

3) Do you provide detailed information on the immunogen

-recombinant human PAX8

4) What is the epitope recognized by the antibody?

-Epitope: aa258-272 of human PAX8.

I hope this is helpful. Please contact me again if you have any further questions.

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Thank you for your patience.

The lab sent me the following information this afternoon:

The ab124445was raised by immunizing full length of human PAX8. The epitope maps to an internal sequence. There is no cross-reactivity with PAX2 and PAX5.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

The immunogen isthe recombinantfull-length human PAX8 protein. The exact epitope has not been mapped, therefore we do not know where the antibody would bind in this region.

As for the specificity and cross-reactivity with Pax2 and Pax5, I will check with the lab for potential data or additional information.

I hope this information is so far helpful to you. I will be in touch. Thank you for your patience.
In the meantime, please do not hesitate to contact us if you need any more advice or information.

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