Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13511] to PAX8 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-PAX8 antibody [EPR13511] - C-terminal
See all PAX8 primary antibodies
DescriptionRabbit monoclonal [EPR13511] to PAX8 - C-terminal
Tested applicationsSuitable for: IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Orangutan
Recombinant full length protein within Human PAX8 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q06710
- IHC-P: Human thyroid carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab189249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionTranscription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Tissue specificityExpressed in the excretory system, thyroid gland and Wilms tumors.
Involvement in diseaseDefects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
Sequence similaritiesContains 1 paired domain.
Developmental stageIn developing excretory system, during thyroid differentiation and in adult thyroid.
- Information by UniProt
- OTTHUMP00000158659 antibody
- OTTHUMP00000158660 antibody
- OTTHUMP00000203723 antibody
Nuclear staining on paraffin-embedded sections of human thyroid carcinoma tissue, performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Samples were incubated with primary antibody at a 1:10000 dilution (0.1 μg/ml). A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as the counterstain. A secondary antibody-only control was also performed.
Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid carcinoma labeling PAX8 with ab189249 at a 1/500 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab189249 has been referenced in 7 publications.
- Tran T et al. In Vivo Developmental Trajectories of Human Podocyte Inform In Vitro Differentiation of Pluripotent Stem Cell-Derived Podocytes. Dev Cell 50:102-116.e6 (2019). PubMed: 31265809
- Li Q et al. Disruption of Robo2-Baiap2 integrated signaling drives cystic disease. JCI Insight 4:N/A (2019). PubMed: 31534052
- Ramalingam H et al. Disparate levels of beta-catenin activity determine nephron progenitor cell fate. Dev Biol 440:13-21 (2018). IF . PubMed: 29705331
- Penner-Goeke S et al. The temporal dynamics of chromosome instability in ovarian cancer cell lines and primary patient samples. PLoS Genet 13:e1006707 (2017). FM ; Human . PubMed: 28376088
- Yamanaka S et al. Generation of interspecies limited chimeric nephrons using a conditional nephron progenitor cell replacement system. Nat Commun 8:1719 (2017). PubMed: 29170512
- Chai HJ et al. PAX8 is a potential marker for the diagnosis of primary epithelial ovarian cancer. Oncol Lett 14:5871-5875 (2017). PubMed: 29113220
- Taylor-Harding B et al. Cyclin E1 and RTK/RAS signaling drive CDK inhibitor resistance via activation of E2F and ETS. Oncotarget 6:696-714 (2015). PubMed: 25557169