• Product name
    Anti-PAX8 antibody [PAX8R1]
    See all PAX8 primary antibodies
  • Description
    Mouse monoclonal [PAX8R1] to PAX8
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, IP, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant C-terminal fragment corresponding to Human PAX8

  • Positive control
    • HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells
  • General notes

    IHC-Fr and IHC-P are not batch-tested and therefore variability between batches might be seen for these applications.



Our Abpromise guarantee covers the use of ab53490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

0.5-2 µg/sample





ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.

1-5 µg/sample

ICC Use a concentration of 2 - 100 µg/ml.
IHC-Fr 1/20.


  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all


  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)

  • ab53490 staining PAX8 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/20 in 10% goat serum in PBS) for 1 hour at 24°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)

  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)

  • IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)

  • ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
  • Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution


This product has been referenced in:
  • Kaku Y  et al. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells. Sci Rep 7:4554 (2017). Read more (PubMed: 28674456) »
  • Liang L  et al. Assessment of the Utility of PAX8 Immunohistochemical Stain in Diagnosing Endocervical Glandular Lesions. Arch Pathol Lab Med 140:148-52 (2016). Read more (PubMed: 26910219) »

See all 10 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Mouse Tissue sections (mouse thyroid)
mouse thyroid
Yes - 0.1% Tween 20

Mr. Sanjay Gawade

Verified customer

Submitted Jun 26 2013

Thank you for contacting us.

I am sorry to hear that you are also experiencing problems with this antibody (additionally to the Anti-TTF1 antibody [8G7G3/1] (ab72876) ). Could you please fill in the questionnaire for both products and send the...

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Thank you for contacting us. Although we have indicated Pax8 to be located in the nucleus, the literature shows Pax8 expression can be nuclear as well as cytoplasmic. I apologize for the confusion.

I hope this information is helpful to you. Pl...

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Thank you for calling Abcam earlier today.

Please find the link to the concentration calculator that we discussed below:


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Thank you for your patience.

I have heard back from the lab with the following information:

We have not done anytesting in regards to cross reactivity of ab53490 with PAX2 and PAX5 on endogenous protein. Also, we haven't done any e...

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Thank you for contacting us.

The immunogen is a recombinant C-terminal fragment corresponding to Human PAX8,based on theamino acid range 318-426 aa. The exact epitope has not been mapped, therefore we do not know where the antibody would bin...

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I'm happy to help! Best of luck with your experiment.

I have used these columns but not for an application as sensitive as the one you describe.For my purposes it had been sufficient to have perhaps 95% or more of protein that should flow through will flow through and about the same percentage of protein ...

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Thank you for your enquiry.

We only have the one formulation of ab53490, which contains 1% BSA. We can often create a custom batch with customer buffer formulations but this would require a bulk order, which would be 10 vials of antibody. Read More

Thank you for contacting us. I am sorry that ab53490 has given no signal on your customer's samples. Since the PAX8 antigen is expressed in the nucleus, adding a brief permeabilization step may help to increase the signal. I would recommend using 0.1...

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