• Product name
    Anti-PAX8 antibody [PAX8R1]
    See all PAX8 primary antibodies
  • Description
    Mouse monoclonal [PAX8R1] to PAX8
  • Host species
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, IP, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant C-terminal fragment corresponding to Human PAX8

  • Positive control
    • HeLa, NIH3T3 or F2408 nuclear cell lysate HeLa cells RIPA lysate of HeLa cells
  • General notes

    IHC-Fr and IHC-P are not batch-tested and therefore variability between batches might be seen for these applications.

    This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab53490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

0.5-2 µg/sample





ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 48 kDa.
IP Use at an assay dependent concentration.

1-5 µg/sample

ICC Use a concentration of 2 - 100 µg/ml.
IHC-Fr 1/20.


  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all


  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ab53490 staining PAX8 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/20 in 10% goat serum in PBS) for 1 hour at 24°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Mouse IgG antibody at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • IHC image of ab53490 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 µg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25µg)

  • ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
  • Immunoprecipitation: ab53490: 5µg, RIPA lysate of HeLa cells: 500µg. WB: ab53490 at a 1:100 dilution, Secondary antibody - anti-mouse IgG at a 1:1000 dilution


This product has been referenced in:
  • Hsu CF  et al. IGF-axis confers transformation and regeneration of fallopian tube fimbria epithelium upon ovulation. EBioMedicine 41:597-609 (2019). Read more (PubMed: 30852161) »
  • Iwadate M  et al. An in vivo model for thyroid regeneration and folliculogenesis. Lab Invest 98:1126-1132 (2018). Read more (PubMed: 29946134) »
See all 14 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Mouse Tissue sections (mouse thyroid)
mouse thyroid
Yes - 0.1% Tween 20

Mr. Sanjay Gawade

Verified customer

Submitted Jun 26 2013

For licensing inquiries, please contact partnerships@abcam.com

Sign up