Overview

  • Product name
    Anti-PAX8 antibody [SP348] - BSA and Azide free
    See all PAX8 primary antibodies
  • Description
    Rabbit monoclonal [SP348] to PAX8 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide within Human PAX8 (N terminal). The exact sequence is proprietary.
    Database link: Q06710

  • Positive control
    • WB: PAX8 transfected HEK-293 cell lysate. IHC-P: Human kidney, uterus, thyroid, fallopian tube, endometrial adenocarcinoma, ovarian adenocarcinoma and renal cell carcinoma tissues. ICC/IF: SK-OV-3 FC: SK-OV-3, HL-60 cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab242429 is a PBS-only buffer format of ab227707. Please refer to ab227707 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab242429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
  • Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
  • Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227707).

References

ab242429 has not yet been referenced specifically in any publications.

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