Overview

  • Product name
    Anti-PAX8 antibody [SP348] - N-terminal
    See all PAX8 primary antibodies
  • Description
    Rabbit monoclonal [SP348] to PAX8 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PAX8 (N terminal).
    Database link: Q06710

  • Positive control
    • WB: PAX8 transfected HEK-293 cell lysate. IHC-P: Human kidney, uterus, thyroid, fallopian tube, endometrial adenocarcinoma, ovarian adenocarcinoma and renal cell carcinoma tissues. ICC/IF: SK-OV-3 FC: SK-OV-3, HL-60 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

WB 1/100. Predicted molecular weight: 48 kDa.

Primary antibody incubation for 1 hour at room temperature.

Target

  • Function
    Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
  • Tissue specificity
    Expressed in the excretory system, thyroid gland and Wilms tumors.
  • Involvement in disease
    Defects in PAX8 are the cause of congenital hypothyroidism non-goitrous type 2 (CHNG2) [MIM:218700]. CHNG2 is a disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue.
  • Sequence similarities
    Contains 1 paired domain.
  • Developmental stage
    In developing excretory system, during thyroid differentiation and in adult thyroid.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000158659 antibody
    • OTTHUMP00000158660 antibody
    • OTTHUMP00000203723 antibody
    • OTTHUMP00000203724 antibody
    • Paired box 8 antibody
    • Paired box gene 8 antibody
    • paired box homeotic gene 8 antibody
    • Paired box protein Pax 8 antibody
    • Paired box protein Pax-8 antibody
    • Paired domain gene 8 antibody
    • PAX 8 antibody
    • PAX8 antibody
    • PAX8_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-PAX8 antibody [SP348] - N-terminal (ab227707) at 1/100 dilution

    Lane 1 : PAX1 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 2 : PAX2 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 3 : PAX3 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 4 : PAX4 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 5 : PAX5 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 6 : PAX6 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 7 : PAX7 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 8 : PAX8 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 9 : PAX9 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
    Lane 10 : Mock transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate

    Predicted band size: 48 kDa

  • Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Patel V  et al. Corded and hyalinized mesonephric-like adenocarcinoma of the uterine corpus: report of a case mimicking endometrioid carcinoma. Hum Pathol 86:243-248 (2019). Read more (PubMed: 30172914) »
See 1 Publication for this product

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