Key features and details
- Rat monoclonal [7C2] to PAX9
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-PAX9 antibody [7C2]
DescriptionRat monoclonal [7C2] to PAX9
SpecificityDoes not cross react with other PAX proteins
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
Recombinant PAX9-MBP fusion protein (Mouse)
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab28538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.|
|Flow Cyt||Use 1µg for 106 cells.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionTranscription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.
Involvement in diseaseDefects in PAX9 are the cause of tooth agenesis selective type 3 (STHAG3) [MIM:604625]. A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth).
Sequence similaritiesContains 1 paired domain.
- Information by UniProt
- Paired box 9 antibody
- Paired box gene 9 antibody
- Paired box homeotic gene 9 antibody
Staining of PAX9 protein in human oesophageal epithelium. Magnification x200
All lanes : Anti-PAX9 antibody [7C2] (ab28538)
Lane 1 : mouse oesophageal protein extract
Lane 2 : human oesophageal protein extract
Predicted band size: 38 kDa
Observed band size: 38 kDa
ICC/IF image of ab28538 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28538, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HepG2 cells stained with ab28538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28538, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was goat anti-rat DyLight® 488 (IgG H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG1, kappa monoclonal [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab28538 has been referenced in 7 publications.
- Bar C et al. Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell Rep 28:257-266.e5 (2019). PubMed: 31269445
- Tata PR et al. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity. Dev Cell 44:679-693.e5 (2018). PubMed: 29587142
- Feng J et al. BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice. Development 144:2560-2569 (2017). PubMed: 28576771
- Sherwood RI et al. Transcriptional dynamics of endodermal organ formation. Dev Dyn 238:29-42 (2009). ICC/IF ; Mouse . PubMed: 19097184
- Kendall J et al. Oncogenic cooperation and coamplification of developmental transcription factor genes in lung cancer. Proc Natl Acad Sci U S A 104:16663-8 (2007). PubMed: 17925434
- Jonker L et al. Pax9 is required for filiform papilla development and suppresses skin-specific differentiation of the mammalian tongue epithelium. Mech Dev 121:1313-22 (2004). PubMed: 15454262
- Gerber JK et al. Progressive loss of PAX9 expression correlates with increasing malignancy of dysplastic and cancerous epithelium of the human oesophagus. J Pathol 197:293-7 (2002). PubMed: 12115874