Product nameAnti-Paxillin antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin
Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Synthetic peptide corresponding to Mouse Paxillin aa 1-100.
(Peptide available as
- This antibody gave a positive signal in NIH3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab2264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22629443|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
ab2264 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.
Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
ab2264 detects a band at 68 kDa in NIH 3T3 lysate, as well as an additional band at 43kDa. An NCBI BLAST search of the peptide sequence identified an N terminal fragment of the Paxillin molecule with a primary molecular weight of 36 kDa. We believe the 43 kDa band observed may correspond to this fragment.
ab2264 staining human and mouse pancreas and rat testis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
Immunohistochemical analysis of frozen sections of frontonasal processes from wild-type (control) or RockDN;Wnt1-cre mutant (RockDN) mouse embyros; Paxillin was stained with ab2264.
This product has been referenced in:
- Neganova I et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). Read more (PubMed: 30512203) »
- Luan L et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). Read more (PubMed: 30186380) »