Overview

  • Product name

  • Description

    Rabbit polyclonal to Paxillin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide corresponding to Mouse Paxillin aa 1-100.
    (Peptide available as ab13754)

  • Positive control

    • This antibody gave a positive signal in NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab2264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 22629443
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

Images

  • ab2264 staining human U2OS, mouse MEF, and rat RIE1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton-100 [PBS] prior to blocking in 1% BSA for 15 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C.  The secondary antibody was an Alexa Fluor® 647 conjugated donkey anti-rabbit, diluted 1/1000.

    See Abreview

  • Anti-Paxillin antibody (ab2264) at 1/500 dilution + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 68 kDa
    Observed band size: 68 kDa
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    ab2264 detects a band at 68 kDa in NIH 3T3 lysate, as well as an additional band at 43kDa. An NCBI BLAST search of the peptide sequence identified an N terminal fragment of the Paxillin molecule with a primary molecular weight of 36 kDa. We believe the 43 kDa band observed may correspond to this fragment.
  • ab2264 staining human and mouse pancreas and rat testis tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C.  The primary antibody was diluted 1/100 and incubated with the sample for 45 minutes at 20°C.  A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of frozen sections of frontonasal processes from wild-type (control) or RockDN;Wnt1-cre mutant (RockDN) mouse embyros; Paxillin was stained with ab2264.

References

This product has been referenced in:

  • Neganova I  et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). Read more (PubMed: 30512203) »
  • Luan L  et al. HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway. Exp Ther Med 16:1621-1628 (2018). Read more (PubMed: 30186380) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-435S)
Specification
MDA-MB-435S
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 28 2013

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS)
Total protein in input
400 µg
Specification
U2OS
Immuno-precipitation step
Protein A

Mr. Antibody Solutions

Verified customer

Submitted Apr 28 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human (U2OS) mouse (MEF) rat (RIE1))
Specification
Human (U2OS) mouse (MEF) rat (RIE1)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-100 [PBS]
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 10 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human and mouse (pancreas), rat (testis))
Specification
human and mouse (pancreas), rat (testis)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Sequential (prediluated) peroxidase and protein block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 08 2008

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS, NIH-3T3, Raw264.7, PC12)
Loading amount
20 µg
Specification
U2OS, NIH-3T3, Raw264.7, PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted Apr 04 2008

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