Product nameAnti-Paxillin antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human Paxillin aa 1-50. The exact sequence is proprietary.
Database link: P49023
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human non-small cell lung cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 65 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||Use at 1-5 µg/mg of lysate.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
All lanes : Anti-Paxillin antibody (ab264125) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Predicted band size: 65 kDa
Exposure time: 10 seconds
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human non-small cell lung cancer tissue labeling Paxillin with ab264125 at 1/200 dilution. Detection: DAB.
Paxillin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264125 at 3ug/mg lysate. Western blot was performed from the immunoprecipitate using ab264125 at 0.04ug/ml.
Lane 1: HeLa whole cell lysate 50ug (Input).
Lane 2: ab264125 IP in HeLa whole cell lysate.
Lane 3: Control IgG.
Exposure time: 10 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264125 has not yet been referenced specifically in any publications.