Product nameAnti-Paxillin antibody [M107]
See all Paxillin primary antibodies
DescriptionMouse monoclonal [M107] to Paxillin
Tested applicationsSuitable for: WB, ELISA, ICC, IPmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Recombinant full length protein (Human).
- A431 cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab23510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa).|
|IP||Use at an assay dependent dilution.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
Lanes 1-2 : Anti-Paxillin antibody [M107] (ab23510)
Lanes 3-4 : Anti-Paxillin (Ser178) antibody
Lanes 1 & 3 : A431 cells (20 ug/lane) serum starved overnight
Lanes 2 & 4 : A431 cells (20 ug/lane) serum treated with EGF (100 ng/ml) for 5 min.
Predicted band size: 65 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
ab23510 has not yet been referenced specifically in any publications.