Product nameAnti-Paxillin antibody [M107]
See all Paxillin primary antibodies
DescriptionMouse monoclonal [M107] to Paxillin
Tested applicationsSuitable for: WB, ELISA, ICC, IPmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
Recombinant full length protein (Human).
- A431 cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab23510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 65 kDa).|
|IP||Use at an assay dependent dilution.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
All lanes : Anti-Paxillin antibody [M107] (ab23510) at 1/1000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : PXN knockout A431 whole cell lysate
Lane 3 : U-87 MG whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 65 kDa
Lanes 1 - 4: Merged signal (red and green). Green - ab23510 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab23510 was shown to recognize PXN in wild-type A431 cells as signal was lost at the expected MW in PXN knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PXN knockout samples were subjected to SDS-PAGE. Ab23510 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Lanes 1-2 : Anti-Paxillin antibody [M107] (ab23510)
Lanes 3-4 : Anti-Paxillin (Ser178) antibody
Lanes 1 & 3 : A431 cells (20 ug/lane) serum starved overnight
Lanes 2 & 4 : A431 cells (20 ug/lane) serum treated with EGF (100 ng/ml) for 5 min.
Predicted band size: 65 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
ab23510 has not yet been referenced specifically in any publications.