Product nameAnti-Paxillin (phospho S126) antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin (phospho S126)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.
- RAW 264.7 cells treated with LPS.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
Our Abpromise guarantee covers the use of ab24402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 68 kDa.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution
Lane 1 : Lysate from untreated RAW 264.7 cells
Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen
All lanes : Goat F(ab)2 anti-rabbit IgG HRP conjugate.
Observed band size: 68 kDa why is the actual band size different from the predicted?
Lysates were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% milk-TBST buffer for one hour at room temperature, and then incubated with the Paxillin [pS126] antibody for two hours at room temperature in a 1% milk-TBST buffer, following its prior incubation with or without peptides. Following incubation with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to Paxillin [pS 126 ] blocks the antibody signal, thereby demonstrating the specificity of the antibody. No competition was seen following incubation with paxillin phosphopeptides to S130, S178, S380, S455, or S479 (not shown). The antibody was also shown to be specific using 293 cells transfected with wild-type EGFP-tagged human paxillin treated with EGF (not shown).