• Product name

    Anti-Paxillin (phospho S126) antibody
    See all Paxillin primary antibodies
  • Description

    Rabbit polyclonal to Paxillin (phospho S126)
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126.

  • Positive control

    • RAW 264.7 cells treated with LPS.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at serine 126.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab24402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa.



  • All lanes : Anti-Paxillin (phospho S126) antibody (ab24402) at 1/1000 dilution

    Lane 1 : Lysate from untreated RAW 264.7 cells
    Lane 2 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes
    Lane 3 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with non-phosphorylated peptide corresponding to the immunogen
    Lane 4 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with generic phosphoserine-containing peptide
    Lane 5 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide corresponding to Paxillin [pS91 ]
    Lane 6 : Lysate from RAW 264.7 cells treated with 1 µg/mL LPS for 60 minutes with phosphopeptide immunogen

    All lanes : Goat F(ab)2 anti-rabbit IgG HRP conjugate.

    Observed band size: 68 kDa
    why is the actual band size different from the predicted?

    Lysates were resolved on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% milk-TBST buffer for one hour at room temperature, and then incubated with the Paxillin [pS126] antibody for two hours at room temperature in a 1% milk-TBST buffer, following its prior incubation with or without peptides. Following incubation with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that only the phosphopeptide corresponding to Paxillin [pS 126 ] blocks the antibody signal, thereby demonstrating the specificity of the antibody. No competition was seen following incubation with paxillin phosphopeptides to S130, S178, S380, S455, or S479 (not shown). The antibody was also shown to be specific using 293 cells transfected with wild-type EGFP-tagged human paxillin treated with EGF (not shown).


This product has been referenced in:

  • Boeckeler K  et al. Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration. J Cell Sci 123:2725-32 (2010). WB ; Rat . Read more (PubMed: 20647370) »
See 1 Publication for this product

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