Overview

  • Product name
    Anti-Paxillin (phospho S83) antibody
    See all Paxillin primary antibodies
  • Description
    Rabbit polyclonal to Paxillin (phospho S83)
  • Host species
    Rabbit
  • Specificity
    ab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide (Mouse) conjugated to KLH corresponding to amino acid residues around serine 83 of paxillin.

  • Positive control
    • Calyculin A treated mouse macrophages.

Properties

Applications

Our Abpromise guarantee covers the use of ab31833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000.
    WB: 1/1000 (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature). Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
    • Sequence similarities
      Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16691 antibody
      • FLJ23042 antibody
      • Paired box protein Pax 1 antibody
      • PAX 1 antibody
      • PAX1 antibody
      • PAXI_HUMAN antibody
      • Paxillin alpha antibody
      • Paxillin antibody
      • PXN antibody
      • PXN protein antibody
      see all

    Images

    • Western blot analysis of immunoprecipitated paxillin (arrow) in mouse macrophages untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min (lanes 2, 4, 5, & 6). Lysates were immunoprecipitated with anti-Paxillin. The blots were probed with anti-Paxillin or anti-phospho-Paxillin (Ser-83). The latter was used in the presence of phospho-Paxillin (Ser-83) peptide (lane 5) or phospho-Paxillin (Ser-85) peptide (lane 6). Immunoglobulin (Ig) chains from the immunoprecipitation were observed above and below the paxillin bands.

    References

    ab31833 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry. This antibody has yet to be tested in human or monkey tissue. The conserved site in human paxillin (Ser-83) has high homology with the mouse paxillin (Ser-83). There is only 2 amino acid differences, however there is evidence that in human the phosphorylation site is Ser-85 rather than Ser-83 (Journal of Cell Biology, (2004) Vol 164(4):593-602.). We have only shown that ab31833 works in mouse, but it may be useful for detecting Ser-83 in human. We currently do not sell the positive control I'm afraid. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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