Key features and details
- Rabbit polyclonal to Paxillin (phospho S83)
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Paxillin (phospho S83) antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin (phospho S83)
Specificityab31833 detects a 68kDa protein corresponding to the molecular mass of phosphorylated paxillin.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide (Mouse) conjugated to KLH corresponding to amino acid residues around serine 83 of paxillin.
- Calyculin A treated mouse macrophages.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 (in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature). Predicted molecular weight: 68 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
Western blot analysis of immunoprecipitated paxillin (arrow) in mouse macrophages untreated (lanes 1 & 3) or treated with calyculin A (100 nM) for 30 min (lanes 2, 4, 5, & 6). Lysates were immunoprecipitated with anti-Paxillin. The blots were probed with anti-Paxillin or anti-phospho-Paxillin (Ser-83). The latter was used in the presence of phospho-Paxillin (Ser-83) peptide (lane 5) or phospho-Paxillin (Ser-85) peptide (lane 6). Immunoglobulin (Ig) chains from the immunoprecipitation were observed above and below the paxillin bands.
ab31833 has not yet been referenced specifically in any publications.