Key features and details
- Rabbit polyclonal to Paxillin (phospho Y118)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Paxillin (phospho Y118) antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin (phospho Y118)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Paxillin (phospho Y118). The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding Y118 of Human Paxillin.
Database link: P49023
- WB: 3T3 cell extracts treated with serum. IHC: Human breast carcinoma tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab194738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
Lane 1 : Anti-Paxillin (phospho Y118) antibody (ab194738) at 1/500 dilution
Lane 2 : Anti-Paxillin (phospho Y118) antibody (ab194738) at 500 cells
Lane 1 : Untreated 3T3 cell extracts
Lane 2 : 3T3 cell extracts treated by serum
Predicted band size: 68 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Paxillin (phospho Y118) with ab194738 at 1/50 dilution.
ab194738 has been referenced in 1 publication.
- Neganova I et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). PubMed: 30512203