Overview

  • Product name

    Anti-Paxillin (phospho Y118) antibody
    See all Paxillin primary antibodies
  • Description

    Rabbit polyclonal to Paxillin (phospho Y118)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Paxillin (phospho Y118). The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding Y118 of Human Paxillin.
    Database link: P49023

  • Positive control

    • WB: 3T3 cell extracts treated with serum. IHC: Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab194738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 68 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function

    Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
  • Sequence similarities

    Belongs to the paxillin family.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ16691 antibody
    • FLJ23042 antibody
    • Paired box protein Pax 1 antibody
    • PAX 1 antibody
    • PAX1 antibody
    • PAXI_HUMAN antibody
    • Paxillin alpha antibody
    • Paxillin antibody
    • PXN antibody
    • PXN protein antibody
    see all

Images

  • Lane 1 : Anti-Paxillin (phospho Y118) antibody (ab194738) at 1/500 dilution
    Lane 2 : Anti-Paxillin (phospho Y118) antibody (ab194738) at 500 cells

    Lane 1 : Untreated 3T3 cell extracts
    Lane 2 : 3T3 cell extracts treated by serum

    Predicted band size: 68 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Paxillin (phospho Y118) with ab194738 at 1/50 dilution.

References

This product has been referenced in:

  • Neganova I  et al. Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency. Stem Cells 37:318-331 (2019). Read more (PubMed: 30512203) »
See 1 Publication for this product

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