Anti-Paxillin (phospho Y118) antibody (ab4833)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y118)
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Paxillin (phospho Y118) antibody
See all Paxillin primary antibodies -
Description
Rabbit polyclonal to Paxillin (phospho Y118) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Chicken -
Immunogen
Synthetic peptide corresponding to Human Paxillin (phospho Y118).
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Positive control
- WB: COS-7, A431, HeLa whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 68 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 68 kDa. |
Target
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Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). -
Sequence similarities
Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > focal adhesion. - Information by UniProt
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Database links
- Entrez Gene: 395832 Chicken
- Entrez Gene: 5829 Human
- Entrez Gene: 19303 Mouse
- Omim: 602505 Human
- SwissProt: P49024 Chicken
- SwissProt: P49023 Human
- SwissProt: Q8VI36 Mouse
- Unigene: 446336 Human
see all -
Alternative names
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
see all
Images
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All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 2 : COS-7 whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : A431 whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : HeLa whole cell lysate treated with 100 uM Pervanadate for 1 hour
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 68 kDaA 66 kDa band corresponding to Paxillin pTyr118 was enhanced across treated cell lines. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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All lanes : Anti-Paxillin (phospho Y118) antibody (ab4833) at 0.75 µg/ml (3% BSA-TBST buffer)
All lanes : NMµMG (mouse mammary) cells transfected with EGFP-tagged paxillin
Secondary
All lanes : goat F(ab’)2 anti-rabbit IgG alkaline phosphatase
Predicted band size: 68 kDaPeptide Competition: Extracts were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC.
Prior incubation with:
1- no peptide,
2- the non-phosphopeptide corresponding to the immunogen
3- a generic phosphotyrosine containing peptide
4- the phosphopeptide immunogen
Bands were detected using the Tropix WesternStar method.
The data show that only the peptide corresponding to ab4833 blocks the antibody signal, thereby demonstrating the specificity of the antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab4833 has been referenced in 6 publications.
- Schimmel L et al. c-Src controls stability of sprouting blood vessels in the developing retina independently of cell-cell adhesion through focal adhesion assembly. Development 147:N/A (2020). PubMed: 32108024
- Zhang M et al. Sea cucumber Cucumaria frondosa fucoidan inhibits osteosarcoma adhesion and migration by regulating cytoskeleton remodeling. Oncol Rep 44:469-476 (2020). PubMed: 32467988
- Wang H et al. Inhibition of tumor vasculogenic mimicry and prolongation of host survival in highly aggressive gallbladder cancers by norcantharidin via blocking the ephrin type a receptor 2/focal adhesion kinase/paxillin signaling pathway. PLoS One 9:e96982 (2014). WB, ICC/IF ; Mouse . PubMed: 24811250
- Lange K et al. Combined lysophosphatidic acid/platelet-derived growth factor signaling triggers glioma cell migration in a tenascin-C microenvironment. Cancer Res 68:6942-52 (2008). WB ; Human . PubMed: 18757408
- Demo SD et al. Antitumor activity of PR-171, a novel irreversible inhibitor of the proteasome. Cancer Res 67:6383-91 (2007). PubMed: 17616698
- Lange K et al. Endothelin receptor type B counteracts tenascin-C-induced endothelin receptor type A-dependent focal adhesion and actin stress fiber disorganization. Cancer Res 67:6163-73 (2007). WB ; Human . PubMed: 17616673