Key features and details
- Rabbit polyclonal to Paxillin (phospho Y118)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Paxillin (phospho Y118) antibody
See all Paxillin primary antibodies
DescriptionRabbit polyclonal to Paxillin (phospho Y118)
Specificityab75740 detects endogenous levels of Paxillin only when phosphorylated at tyrosine 118.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).
- Human breast carcinoma tissue. Extracts from HeLa cells treated with EGF.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75740 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab75740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
ab75740 at 1/50 dilution staining Paxillin in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
All lanes : Anti-Paxillin (phospho Y118) antibody (ab75740) at 1/500 dilution
Lane 1 : HeLa cell extract, untreated
Lane 2 : HeLa cell extract treated with EGF
Predicted band size: 64 kDa
Observed band size: 64 kDa
ab75740 has been referenced in 2 publications.
- Alsaffar H et al. Interleukin-6 promotes a sustained loss of endothelial barrier function via Janus kinase-mediated STAT3 phosphorylation and de novo protein synthesis. Am J Physiol Cell Physiol 314:C589-C602 (2018). PubMed: 29351406
- Adam AP et al. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-a: Crosstalk with p38 Signaling. PLoS One 11:e0161975 (2016). PubMed: 27603666