Product nameAnti-Paxillin (phospho Y181) antibody [EP41(2)Y]
See all Paxillin primary antibodies
DescriptionRabbit monoclonal [EP41(2)Y] to Paxillin (phospho Y181)
Specificityab76235 only detects Paxillin phosphorylated on Tyrosine 181.
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic phosphopeptide corresponding to residues surrounding Tyrosine 181 of Human Paxillin.
- HeLa cell lysate treated with pervanadate.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Sequence similaritiesBelongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
modificationsPhosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > focal adhesion.
- Information by UniProt
- FLJ16691 antibody
- FLJ23042 antibody
- Paired box protein Pax 1 antibody
All lanes : Anti-Paxillin (phospho Y181) antibody [EP41(2)Y] (ab76235) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Yanagishita T et al. Actin-binding protein, Espin: a novel metastatic regulator for melanoma. Mol Cancer Res 12:440-6 (2014). WB ; Mouse . Read more (PubMed: 24337068) »
- Kim SJ et al. Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). PLoS One 6:e25103 (2011). WB ; Human . Read more (PubMed: 21966428) »